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https://doi.org/10.1016/j.molimm.2016.02.012
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Título: | A novel CD14high CD16high subset of peritoneal macrophages from cirrhotic patients is associated to an increased response to LPS |
Fecha de publicación: | 1-mar-2016 |
Editorial: | Elsevier |
Cita bibliográfica: | Molecular Immunology 72 (2016) 28–36 |
ISSN: | Print: 0161-5890 Electronic: 1872-9142 |
Palabras clave: | Cirrhosis Ascites Inflammation Monocyte subsets Cell signaling Cytokines |
Resumen: | The aim of this study was to characterize monocyte-derived macrophages (M-DM) from blood and ascites of cirrhotic patients comparatively with those obtained from blood of healthy controls. The phenotypic profile based on CD14/CD16 expression was analyzed by flow cytometry. Cells were isolated and stimulated in vitro with LPS and heat killed Candida albicans. Phosphorylation of ERK, c-Jun, p38 MAPK, and PKB/Akt was analyzed by Western blotting. A novel CD14(high)CD16(high) M-DM subpopulation is present in ascites (∼33%). The CD14(++)CD16(+) intermediate subset is increased in the blood of cirrhotic patients (∼from 4% to 11%) and is predominant in ascites (49%), while the classical CD14(++)CD16(-) subpopulation is notably reduced in ascites (18%). Basal hyperactivation of ERK and JNK/c-Jun pathways observed in ascites M-DM correlates with CD14/CD16 high expressing subsets, while PI3K/PKB does it with the CD16 low expressing cells. In vitro LPS treatment highly increases ERK1/2, PKB/Akt and c-Jun phosphorylation, while that of p38 MAPK is decreased in M-DM from ascites compared to control blood M-DM. Stimulation of healthy blood M-DM with LPS and C. albicans induced higher phosphorylation levels of p38 than those from ascites. Regarding cytokines secretion, in vitro activated M-DM from ascites of cirrhotic patients produced significantly higher amounts of IL-6, IL-10 and TNF-α, and lower levels of IL-1β and IL-12 than control blood M-DM. In conclusion, a new subpopulation of CD14(high)CD16(high) peritoneal M-DM has been identified in ascites of cirrhotic patients, which is very sensitive to LPS stimulation. |
Autor/es principal/es: | Martínez-Esparza, M. Ruiz-Alcaraz, Antonio José Tapia-Abellán, Ana Fernández-Fernández, María Dolores Tristán-Manzano, María Hernández-Caselles, Trinidad Sánchez-Velasco, Eduardo Miras-López, Manuel García-Penarrubia, Pilar |
Facultad/Departamentos/Servicios: | Facultades, Departamentos, Servicios y Escuelas::Departamentos de la UMU::Bioquímica y Biología Molecular B e Inmunología |
Versión del editor: | https://www.sciencedirect.com/science/article/pii/S0161589016300256?via%3Dihub |
URI: | http://hdl.handle.net/10201/142789 |
DOI: | https://doi.org/10.1016/j.molimm.2016.02.012 |
Tipo de documento: | info:eu-repo/semantics/article |
Número páginas / Extensión: | 9 |
Derechos: | info:eu-repo/semantics/embargoedAccess |
Descripción: | © 2016 Elsevier Ltd. All rights reseved. This document is the Published version of a Published Work that appeared in final form in Molecular Immunology. To access the final edited and published work see https://doi.org/10.1016/j.molimm.2016.02.012 |
Aparece en las colecciones: | Artículos: Bioquímica y Biología Molecular "B" e Inmunología |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
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Mol Immunol cirrosis 2016.pdf | 2,35 MB | Adobe PDF | Visualizar/Abrir Solicitar una copia |
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