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https://doi.org/10.1111/imcb.12305
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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Ruiz Alcaraz, Antonio José | - |
dc.contributor.author | Martínez Banaclocha, Helios | - |
dc.contributor.author | Marín Sánchez, Pilar | - |
dc.contributor.author | Carmona Martínez, Violeta | - |
dc.contributor.author | Iniesta Albadalejo, Miguel Ángel | - |
dc.contributor.author | Tristán Manzano, María | - |
dc.contributor.author | Tapia Abellán, Ana | - |
dc.contributor.author | García Peñarrubia, Pilar | - |
dc.contributor.author | Machado Linde, Francisco | - |
dc.contributor.author | Pelegrín, Pablo | - |
dc.contributor.author | Martínez Esparza, M. | - |
dc.contributor.other | Facultades, Departamentos, Servicios y Escuelas::Departamentos de la UMU::Bioquímica y Biología Molecular B e Inmunología | es |
dc.date.accessioned | 2024-06-26T11:40:14Z | - |
dc.date.available | 2024-06-26T11:40:14Z | - |
dc.date.issued | 2019-11-06 | - |
dc.identifier.citation | Immunology & Cell Biology, 2020, Vol. 98, N. 2, pp. 114-126 | es |
dc.identifier.issn | Print: 0818-9641 | - |
dc.identifier.issn | Electronic: 1440-1711 | - |
dc.identifier.uri | http://hdl.handle.net/10201/142711 | - |
dc.description | © 2019 Australian and New Zealand Society for Immunology Inc. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ This document is the Accepted version of a Published Work that appeared in final form in Immunology & Cell Biology. To access the final edited and published work see https://doi.org/10.1111/imcb.12305 | - |
dc.description.abstract | Macrophages play an important role in the inflammatory response. Their various biological functions are induced by different membrane receptors, including Toll-like receptors, which trigger several intracellular signaling cascades and activate the inflammasomes, which in turn elicit the release of inflammatory mediators such as cytokines. In this study, we present a novel method for the isolation of human mature peritoneal macrophages. This method can be easily implemented by gynecologists who routinely perform laparoscopy for sterilization by tubal ligation or surgically intervene in benign gynecological pathologies. Our method confirms that macrophages are the main peritoneal leukocyte subpopulation isolated from the human peritoneum in homeostasis. We showed that primary human peritoneal macrophages present phagocytic and oxidative activities, and respond to activation of the main proinflammatory pathways such as Toll-like receptors and inflammasomes, resulting in the secretion of different proinflammatory cytokines. Therefore, this method provides a useful tool for characterizing primary human macrophages as control cells for studies of molecular inflammatory pathways in steady-state conditions and for comparing them with those obtained from pathologies involving the peritoneal cavity. Furthermore, it will facilitate advances in the screening of anti-inflammatory compounds in the human system. | es |
dc.format | application/pdf | es |
dc.format.extent | 13 | es |
dc.language | eng | es |
dc.publisher | Wiley | es |
dc.relation | The study was supported by the Precipita crowdfunding platform (PR200; Endometriosis to PG-P), Fundación Española para la Ciencia y la Tecnología Ministerio de Ciencia y Universidades, Spain, the Ministerio de Economía, Industria y Competitividad (SAF2017-88276-R to PP), the Fundación Séneca (20859/PI/18 to PP) and the European Research Council (ERC-2013-CoG 614578 to PP). HM-B was supported by a Rio Hortega fellowship from the Instituto Salud Carlos III (CM14/00008). VC-M was supported by the Dirección General de Investigación Científica y Técnica and Fondo Social Europeo (EJ-2014-A-95803), Spain. | es |
dc.rights | info:eu-repo/semantics/openAccess | es |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Cytokines | es |
dc.subject | Human macrophages | es |
dc.subject | Inflammation | es |
dc.subject | Innate immunity | es |
dc.subject | Peritoneal leukocytes | es |
dc.title | Isolation of functional mature peritoneal macrophages from healthy humans. | es |
dc.type | info:eu-repo/semantics/article | es |
dc.relation.publisherversion | https://onlinelibrary.wiley.com/doi/10.1111/imcb.12305 | es |
dc.identifier.doi | https://doi.org/10.1111/imcb.12305 | - |
Aparece en las colecciones: | Artículos: Bioquímica y Biología Molecular "B" e Inmunología |
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16618530.pdf | 1,48 MB | Adobe PDF | ![]() Visualizar/Abrir |
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