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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Núñez, Andrés | - |
dc.contributor.author | Dulude, Dominic | - |
dc.contributor.author | Jbel, Mehdi | - |
dc.contributor.author | Rokeach, Luis A. | - |
dc.contributor.other | Facultades, Departamentos, Servicios y Escuelas::Departamentos de la UMU::Genética y Microbiología | es |
dc.date.accessioned | 2024-06-17T07:57:42Z | - |
dc.date.available | 2024-06-17T07:57:42Z | - |
dc.date.issued | 2015-03-24 | - |
dc.identifier.citation | PLoS ONE 10(3): e0121059 | es |
dc.identifier.issn | Electronic: 1932-6203 | - |
dc.identifier.uri | http://hdl.handle.net/10201/142375 | - |
dc.description | ©2015 2015 Núñez et al. This manuscript version is made available under the CC-BY 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/. This document is the Published, version of a Published Work that appeared in final form in PLoS ONE . To access the final edited and published work see https://doi.org/10.1371/journal.pone.0121059 | - |
dc.description.abstract | Cell fate is determined by the balance of conserved molecular mechanisms regulating death (apoptosis) and survival (autophagy). Autophagy is a process by which cells recycle their organelles and macromolecules through degradation within the vacuole in yeast and plants, and lysosome in metazoa. In the yeast Schizosaccharomyces pombe, autophagy is strongly induced under nitrogen starvation and in aging cells. Previously, we demonstrated that calnexin (Cnx1p), a highly conserved transmembrane chaperone of the endoplasmic reticulum (ER), regulates apoptosis under ER stress or inositol starvation. Moreover, we showedthat in stationary phase, Cnx1p is cleaved into two moieties, L_Cnx1p and S_Cnx1p. Here, weshowthattheprocessing of Cnx1p is regulated by autophagy, induced by nitrogen starvation or cell aging. The cleavage of Cnx1p involves two vacuolar proteases: Isp6, which is essential for autophagy, and its paralogue Psp3. Blocking autophagy through the knockout of autophagy-related genes (atg) results in inhibition of both, the cleavage and the trafficking of Cnx1p from the ER to the vacuole. We demonstrate that Cnx1pis required for cell survival under nitrogen-starvation and in chronological aging cultures. The death of the mini_cnx1 mutant (overlapping S_cnx1p) cells is accompanied by accumulation of high levels of reactive-oxygen species (ROS), a slowdown in endocytosis and severe cell-wall defects. Moreover, mutant cells expressing only S_Cnx1p showed cell wall defects. Co-expressing mutant overlapping the L_Cnx1p and S_Cnx1p cleavage products reverses the death, ROS phenotype and cell wall defect to wild-type levels. As it is involved in both apoptosis and autophagy, Cnx1p could be a nexus for the crosstalk between these pro-death and pro-survival mechanisms. Ours, and observations in mammalian systems, suggest that the multiple roles of calnexin depend on its sub-cellular localization and onits cleavage. The use of S. pombeshouldassist in further shedding light on the multiple roles of calnexin. | es |
dc.format | application/pdf | es |
dc.format.extent | 27 | es |
dc.language | eng | es |
dc.publisher | Public Library of Science | es |
dc.relation | Trabajo financiado por GI MOP 89702, MOP-119481 (Canadian Institutes for Health Research (CIHR)), National Sciences and Engineering Council of Canada 171325 concedido a LAR; Beca postdoctoral concedida a Andrés Núñez por la Fundación Séneca-Región de Murcia "Plan de Ciencia, Tecnología e Innovación de la Región de Murcia (2011-2014) | es |
dc.rights | info:eu-repo/semantics/openAccess | es |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights | Attribution-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nd/4.0/ | * |
dc.subject | Microbiology | es |
dc.subject | Autophagy | es |
dc.subject | Calnexin | es |
dc.subject | Apoptosis | es |
dc.subject | Cell signaling | es |
dc.subject.other | CDU::5 - Ciencias puras y naturales::57 - Biología::579 - Microbiología | es |
dc.subject.other | CDU::5 - Ciencias puras y naturales::57 - Biología::576 - Biología celular y subcelular. Citología | es |
dc.title | Calnexin is essential for survival under nitrogen starvation and stationary phase in Schizosaccharomyces pombe | es |
dc.type | info:eu-repo/semantics/article | es |
dc.identifier.doi | https://doi.org/10.1371/journal.pone.0121059 | - |
Aparece en las colecciones: | Artículos: Genética y Microbiología |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
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Calnexin.pdf | Calnexin_2015 | 2,2 MB | Adobe PDF | Visualizar/Abrir |
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