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https://doi.org/10.1016/j.exger.2018.08.010
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Título: | Impact of a shift work-like lighting schedule on the functioning of the circadian system in the short-lived fish Nothobranchius furzeri |
Fecha de publicación: | 2-sep-2018 |
Editorial: | Elsevier |
Cita bibliográfica: | Experimental Gerontology 112 (2018) 44–53 |
ISSN: | Print: 0531-5565 |
Palabras clave: | Circadian rhythm Chronodisruption Nothobranchius Shift-work Molecular clock |
Resumen: | Adult Nothobranchius furzeri of the MZM-04/10 strain were individually kept and subjected to a “5+2” shifting lighting schedule (SHIFT) for 8 weeks in order to evaluate the desynchronizing effects of a simulated human-like shift-work schedule on the functioning of the circadian system (CS). With this aim, sixteen 21-week-old N. furzeri were placed into a Morning, Night and Evening schedule (lights on from 08:00 to 16:00, 00:00 to 08:00 and 16:00 to 00:00 h, respectively) and fed once a day in the middle of the corresponding photophase (12:00, 04:00 and 20:00 h, respectively). Then, in the weekends (2 days), fish were always returned to the Morning shift. As controls, 16 fish were maintained under a non-shifting LD cycle condition (CONTROL) throughout the whole experiment, with lights on from 08:00 to 16:00 h. Rest-activity rhythm (RAR) of fish subjected to SHIFT showed several symptoms of chronodisruption, such as a decrease in the percentage of diurnal activity and a reduction of the relative amplitude and the circadian function index with time. When a periodogram analysis was performed, RAR of N. furzeri under SHIFT conditions showed up to three separate circadian components: one longer than 24 h (26.5 h) that followed the weekly 8 h delays; a short-period component (~23 h) that was related to the weekend's phase advances, and finally, a 24 h component. The shifting LD schedule also affected fish CS at a molecular level, with several significant differences in the expression of core genes of the molecular clock (bmal1, clock, rorα, rev-erbα) between SHIFT and CONTROL animals. RAR impairment along with changes in clock gene expression could be associated with high stress and accelerated aging in these fish. |
Autor/es principal/es: | Almaida Pagán, Pedro Francisco Ortega-Sabater, C. Lucas-Sánchez, A. Martinez-Nicolas, A. Espinosa, C. Esteban, M. A. Madrid, J. A. Rol, M. Mendiola, P. Costa, J. de |
Facultad/Departamentos/Servicios: | Facultades, Departamentos, Servicios y Escuelas::Departamentos de la UMU::Fisiología |
Versión del editor: | https://www.sciencedirect.com/journal/experimental-gerontology/vol/112/suppl/C |
URI: | http://hdl.handle.net/10201/142356 |
DOI: | https://doi.org/10.1016/j.exger.2018.08.010 |
Tipo de documento: | info:eu-repo/semantics/article |
Número páginas / Extensión: | 9 |
Derechos: | info:eu-repo/semantics/embargoedAccess |
Descripción: | © 2018 Elsevier Inc. All rights reserved. This document is the Published version of a Published Work that appeared in final form in Experimental Gerontology. To access the final edited and published work see https://doi.org/10.1016/j.exger.2018.08.010 |
Aparece en las colecciones: | Artículos: Fisiología |
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16.Almaida-Pagán et al., 2018.pdf | 1,44 MB | Adobe PDF | Visualizar/Abrir Solicitar una copia |
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