Por favor, use este identificador para citar o enlazar este ítem:
https://doi.org/10.3390/ijms241310948
![](/digitum/image/email_logo.png)
![](/digitum/image/logo-facebook.png)
Registro completo de metadatos
Campo DC | Valor | Lengua/Idioma |
---|---|---|
dc.contributor.author | Romecín, Paola | - |
dc.contributor.author | García Estañ López, Joaquín María | - |
dc.contributor.author | Marin Atucha, Noemí | - |
dc.contributor.author | Akbari Aghdam, Masoud | - |
dc.contributor.other | Facultades, Departamentos, Servicios y Escuelas::Departamentos de la UMU::Fisiología | - |
dc.date.accessioned | 2024-04-18T22:12:09Z | - |
dc.date.available | 2024-04-18T22:12:09Z | - |
dc.date.issued | 2023-06-30 | - |
dc.identifier.citation | International Journal of Molecular Sciences, 2023, 24, 10948 | es_ES |
dc.identifier.issn | Print: 1661-6596 | - |
dc.identifier.issn | Electronic: 1422-0067 | - |
dc.identifier.uri | http://hdl.handle.net/10201/140914 | - |
dc.description | ©2023. This manuscript version is made available under the CC-BY 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ This document is the Published, version of a Published Work that appeared in final form in International Journal of Molecular Sciences . To access the final edited and published work see https://doi.org/10.3390/ijms241310948 | - |
dc.description.abstract | : Introduction: Previously, we found that intracellular calcium (Ca2+) homeostasis is altered in platelets from an experimental model of liver cirrhosis, namely the bile-duct-ligated (BDL) rat. These alterations are compatible with the existence of a hypercoagulable state. Objective: In the present study, we analyzed the role of nitric oxide in the abnormal calcium signaling responses of an experimental cirrhosis model, the bile duct-ligated rat. Methods: Chronic treatment with LNAME was used to inhibit NO production in a group of control and BDL animals, and the responses compared to those obtained in a control and BDL untreated group (n = 6 each). The experiments were conducted on isolated platelets loaded with fura-2, using fluorescence spectrometry. Results: Chronic treatment with L-NAME increased thrombin-induced Ca2+ release from internal stores in both control and BDL rats. However, the effect was significantly greater in the BDL rats (p < 0.05). Thrombin-induced calcium entry from the extracellular space was also elevated but at lower doses and, similarly in both control and BDL platelets, treated with the NO synthesis inhibitor. Capacitative calcium entry was also enhanced in the control platelets but not in platelets from BDL rats treated with L-NAME. Total calcium in intracellular stores was elevated in untreated platelets from BDL rats, and L-NAME pretreatment significantly (p < 0.05) elevated these values both in controls and in BDL but significantly more in the BDL rats (p < 0.05). Conclusions: Our results suggest that nitric oxide plays a role in the abnormal calcium signaling responses observed in platelets from BDL rats by interfering with the mechanism that releases calcium from the internal stores. | - |
dc.format | application/pdf | es_ES |
dc.format.extent | 8 | - |
dc.language | eng | es_ES |
dc.publisher | MDPI | - |
dc.relation | The experiments were performed thanks to grants from Ministry of Science, Fundacion Seneca and Campus Mare Nostrum of the Universidad de Murcia. | - |
dc.rights | info:eu-repo/semantics/openAccess | * |
dc.rights | Atribución 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject | Nitric oxide | es_ES |
dc.subject | Platelets | es_ES |
dc.subject | Calcium signaling | - |
dc.subject | Bile-duct ligation | - |
dc.subject | Capacitative Ca2+ entry | - |
dc.subject | Liver cirrhosis | - |
dc.subject | Fura-2 | - |
dc.subject | Thapsigargin | - |
dc.subject | Thrombin | - |
dc.title | Role of Nitric Oxide in the Altered Calcium Homeostasis of Platelets from Rats with Biliary Cirrhosis | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.relation.publisherversion | https://www.mdpi.com/1422-0067/24/13/10948 | - |
dc.identifier.doi | https://doi.org/10.3390/ijms241310948 | - |
Aparece en las colecciones: | Artículos: Fisiología |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
ijms-24-10948-v2.pdf | 1,13 MB | Adobe PDF | ![]() Visualizar/Abrir |
Este ítem está sujeto a una licencia Creative Commons Licencia Creative Commons