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https://doi.org/ 10.1128/JVI.66.5.2748-2753.1992
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Título: | Recombinant Vaccine for Canine Parvovirus in Dogs |
Fecha de publicación: | 7-feb-1992 |
Editorial: | American Society for Microbiology |
Cita bibliográfica: | Journal of Virology 66(5):2748-53 |
ISSN: | Print: 0022-538X Electronic: 1098-5514 |
Resumen: | VP2 is the major component of canine parvovirus (CPV) capsids. The VP2-coding gene was engineered to be expressed by a recombinant baculovirus under the control of the polyhedrin promoter. A transfer vector that contains the lacZ gene under the control of the plO promoter was used in order to facilitate the selection of recombinants. The expressed VP2 was found to be structurally and immunologically indistinguishable from authentic VP2. The recombinant VP2 shows also the capability to self-assemble, forming viruslike particles similar in size and appearance to CPV virions. These viruslike particles have been used to immunize dogs in different doses and combinations of adjuvants, and the anti-CPV responses have been measured by enzyme-linked immunosorbent assay, monolayer protection assays, and an assay for the inhibition of hemagglutination. A dose of ca. 10 ,ug of VP2 was able to elicit a good protective response, higher than that obtained with a commercially available, inactivated vaccine. The results indicate that these viruslike particles can be used to protect dogs from CPV infection. |
Autor/es principal/es: | López de Turiso, José Ángel Cortés, Elena Martínez, Concepción Ruiz de Ybáñez Carnero, María del Rocío Simarro, Isabel Vela, Carmen Casal, Ignacio |
Facultad/Departamentos/Servicios: | Facultades, Departamentos, Servicios y Escuelas::Departamentos de la UMU::Sanidad Animal |
Versión del editor: | https://journals.asm.org/doi/epdf/10.1128/jvi.66.5.2748-2753.1992 |
URI: | http://hdl.handle.net/10201/140647 |
DOI: | https://doi.org/ 10.1128/JVI.66.5.2748-2753.1992 |
Tipo de documento: | info:eu-repo/semantics/article |
Número páginas / Extensión: | 6 |
Derechos: | info:eu-repo/semantics/openAccess Attribution-NonCommercial-NoDerivatives 4.0 Internacional |
Descripción: | ©1992,American Society for Microbiology. This manuscript version is made available under the CC-BY NC/ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/. This document is the Published, version of a Published Work that appeared in final form in Journal of Virology. To access the final edited and published work see https://doi.org/ 10.1128/JVI.66.5.2748-2753.1992 |
Aparece en las colecciones: | Artículos: Sanidad Animal |
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Recombinant vaccine for canine parvovirus in dogs.pdf | 1,61 MB | Adobe PDF | Visualizar/Abrir |
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