Por favor, use este identificador para citar o enlazar este ítem:
https://doi.org/10.1371/journal.pone.0172574
Twittear
Registro completo de metadatos
Campo DC | Valor | Lengua/Idioma |
---|---|---|
dc.contributor.author | Bernabé García, Ángel | - |
dc.contributor.author | Armero Barranco, David | - |
dc.contributor.author | Liarte, Sergio | - |
dc.contributor.author | Ruzafa Martínez, María | - |
dc.contributor.author | Ramos Morcillo, Antonio Jesús | - |
dc.contributor.author | Nicolás, Francisco José | - |
dc.contributor.other | Facultades, Departamentos, Servicios y Escuelas::Departamentos de la UMU::Enfermería | es |
dc.date.accessioned | 2024-02-28T11:37:09Z | - |
dc.date.available | 2024-02-28T11:37:09Z | - |
dc.date.issued | 2017-02-23 | - |
dc.identifier.citation | PLoS ONE 12(2) 2017: e0172574 | es |
dc.identifier.issn | Electronic: 1932-6203 | - |
dc.identifier.uri | http://hdl.handle.net/10201/139708 | - |
dc.description | ©2017. This manuscript version is made available under the CC-BY 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ This document is the Published, version of a Published Work that appeared in final form in PLoS ONE. To access the final edited and published work see https://doi.org/10.1371/journal.pone.0172574 | es |
dc.description.abstract | During wound healing, skin function is restored by the action of several cell types that undergo differentiation, migration, proliferation and/or apoptosis. These dynamics are tightly regulated by the evolution of the extra cellular matrix (ECM) contents along the process. Pharmacologically active flavonoids have shown to exhibit useful physiological properties interesting in pathological states. Among them, oleanolic acid (OA), a pentacyclic triterpene, shows promising properties over wound healing, as increased cell migration in vitro and improved wound resolution in vivo. In this paper, we pursued to disclose the molecular mechanisms underlying those effects, by using an in vitro scratch assay in two epithelial cell lines of different linage: non-malignant mink lung epithelial cells, Mv1Lu; and human breast cancer cells, MDA-MB-231. In every case, we observed that OA clearly enhanced cell migration for in vitro scratch closure. This correlated with the stimulation of molecular pathways related to mitogen-activated protein (MAP) kinases, as ERK1,2 and Jun N-terminal kinase (JNK) 1,2 activation and c-Jun phosphorylation. Moreover, MDA-MB-231 cells treated with OA displayed an altered gene expression profile affecting transcription factor genes (c-JUN) as well as proteins involved in migration and ECM dynamics (PAI1), in line with the development of an epithelial to mesenchymal transition (EMT) status. Strikingly, upon OA treatment, we observed changes in the epidermal growth factor receptor (EGFR) subcellular localization, while interfering with its signalling completely prevented migration effects. This data provides a physiological framework supporting the notion that lipophilic plant extracts used in traditional medicine, might modulate wound healing processes in vivo through its OA contents. The molecular implications of these observations are discussed. | es |
dc.format | application/pdf | es |
dc.format.extent | 24 | es |
dc.language | eng | es |
dc.publisher | Public Library of Science | es |
dc.relation | Sin financiación externa a la Universidad | es |
dc.rights | info:eu-repo/semantics/openAccess | es |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject.other | CDU::6 - Ciencias aplicadas::61 - Medicina::614 - Higiene y salud pública. Contaminación. Prevención de accidentes. Enfermería | es |
dc.title | Oleanolic acid induces migration in Mv1Lu and MDA-MB-231 epithelial cells involving EGF receptor and MAP kinases activation | es |
dc.type | info:eu-repo/semantics/article | es |
dc.identifier.doi | https://doi.org/10.1371/journal.pone.0172574 | - |
Aparece en las colecciones: | Artículos: Enfermería |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
2017 OLEANOLICO plos one.pdf | 5,12 MB | Adobe PDF | Visualizar/Abrir |
Este ítem está sujeto a una licencia Creative Commons Licencia Creative Commons