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https://doi.org/10.1016/j.theriogenology.2019.05.045
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Título: | Pig in vitro fertilization: where are we and where do we go? |
Fecha de publicación: | 2019 |
Fecha de defensa / creación: | 2019 |
Cita bibliográfica: | Theriogenology |
ISSN: | 0093-691X 1879-3231 |
Materias relacionadas: | CDU::6 - Ciencias aplicadas::61 - Medicina::612 - Fisiología |
Palabras clave: | Pig In vitro fertilization Efficiency Additives Monospermy In vitro production |
Resumen: | The pig is an important livestock animal. Biotechnological interest in this species has increased due to its use, among others, in the generation of transgenic animals for use in biomedicine based on its greater physiological proximity to the human species than other large domestic animals. This development has paralleled an improvement in Assisted Reproduction Techniques (ART) used for this species. However, the ability to generate animals from embryos produced entirely in vitro is still limited and a wide margin for improvement remains. Here we review the procedures, additives, and devices used during pig in vitro fertilization (IVF), focusing on the main points of each step that have offered the best results in terms of increased efficiency of the system. The lack of standardized protocols and consensus on the parameters to be assessed makes it difficult to compare results across different studies, but some conclusions are drawn from the literature. We anticipate that new physiological protocols will advance the field of swine IVF, including induction of prefertilization ZP hardening with oviductal fluid, sperm preparation by swim-up method, increased viscosity through the addition of inert molecules or reproductive biofluids, and the incorporation of 3D devices. Here we also reflect on the need to expand the variables on which the efficiency of pig IVF is based, providing new parameters that should be considered to supply more objective and quantitative assessment of IVF additives and protocols. |
Autor/es principal/es: | Romar, Raquel Cánovas, Sebastián Matás, Carmen Gadea, Joaquín Coy, Pilar |
Facultad/Departamentos/Servicios: | Facultades, Departamentos, Servicios y Escuelas::Departamentos de la UMU::Fisiología |
Versión del editor: | https://www.sciencedirect.com/science/article/pii/S0093691X19301840 |
URI: | http://hdl.handle.net/10201/139287 |
DOI: | https://doi.org/10.1016/j.theriogenology.2019.05.045 |
Tipo de documento: | info:eu-repo/semantics/article |
Número páginas / Extensión: | 48 |
Derechos: | info:eu-repo/semantics/openAccess Attribution-NonCommercial-NoDerivatives 4.0 Internacional |
Descripción: | ©2019. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ This document is the Accepted version of a Published Work that appeared in final form in Theriogenology. To access the final edited and published work see https://doi.org/10.1016/j.theriogenology.2019.05.045 |
Aparece en las colecciones: | Artículos: Fisiología |
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Fichero | Descripción | Tamaño | Formato | |
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Romar, Canovas et al., 2019 Theriogenology, Accepted manuscript.pdf | 1,74 MB | Adobe PDF | Visualizar/Abrir |
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