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https://doi.org/10.1093/cvr/cvab197
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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Rouhi, Leila | - |
dc.contributor.author | Fan, Siyang | - |
dc.contributor.author | Cheedipudi, Sirisha M. | - |
dc.contributor.author | Braza Boils, Aitana | - |
dc.contributor.author | Sabater Molina, María | - |
dc.contributor.author | Yao, Yan | - |
dc.contributor.author | Robertson, Matthew J. | - |
dc.contributor.author | Coarfa, Cristian | - |
dc.contributor.author | Gimeno, Juan Ramón | - |
dc.contributor.author | Molina, Pilar | - |
dc.contributor.author | Gurha, Priyatansh | - |
dc.contributor.author | Zorio, Esther | - |
dc.contributor.author | Marian, Ali J. | - |
dc.contributor.other | Facultades, Departamentos, Servicios y Escuelas::Departamentos de la UMU::Ciencias Sociosanitarias | - |
dc.date.accessioned | 2024-02-12T12:05:21Z | - |
dc.date.available | 2024-02-12T12:05:21Z | - |
dc.date.issued | 2021-06-14 | - |
dc.identifier.citation | Cardiovascular Research; 118(6) 2022: 1466-1478 | es |
dc.identifier.issn | Print: 0008-6363 | - |
dc.identifier.issn | Electronic: 1755-3245 | - |
dc.identifier.uri | http://hdl.handle.net/10201/139286 | - |
dc.description | ©2022. This document is the Published version of a Published Work that appeared in final form in Cardiovascular Research. To access the final edited and published work see https://doi.org/10.1093/cvr/cvab197 | - |
dc.description.abstract | Aims Arrhythmogenic cardiomyopathy (ACM) is a primary myocardial disease that typically manifests with cardiacarrhythmias, progressive heart failure, and sudden cardiac death (SCD). ACM is mainly caused by mutations ingenes encoding desmosome proteins. Desmosomes are cell–cell adhesion structures and hubs for mechanosensing and mechanotransduction. The objective was to identify the dysregulated molecular and biological pathways in human ACM in the absence of overt heart failure. Methods and results Transcriptomes in the right ventricular endomyocardial biopsy samples from three independent individuals carrying truncating mutations in the DSP gene and five control samples were analysed by RNA-Seq (discovery group). These cases presented with cardiac arrhythmias and had a normal right ventricular function. The RNA-Seq analysis identified 5000 differentially expressed genes (DEGs), which predicted suppression of the Hippo and canonical WNT pathways, among others. Dysregulated genes and pathways, identified by RNA-Seq, were tested for validation in the right and left ventricular tissues from five independent autopsy-confirmed ACM cases with defined mutations (validation group), who were victims of SCD and had no history of heart failure. Protein levels and nuclear localization of the cWNT and Hippo pathway transcriptional regulators were reduced in the right and left ventricular validation samples. In contrast, levels of acetyltransferase EP300, known to suppress the Hippo and canonical WNT pathways, were increased and its bona fide target TP53 was acetylated. RNA-Seq data identified apical junction, reflective of cell–cell attachment, as the most disrupted biological pathway, which were corroborated by disrupted desmosomes and intermediate filament structures. Moreover, the DEGs also predicted dysregulation of over a dozen canonical signal transduction pathways, including the Tec kinase and integrin signalling pathways. The changes were associated with increased apoptosis and fibro-adipogenesis in the ACM hearts. | es |
dc.format | application/pdf | es |
dc.format.extent | 13 | es |
dc.language | eng | es |
dc.publisher | Oxford University Press [University Publisher] | - |
dc.publisher | European Society of Cardiology [Society Publisher] | - |
dc.relation | The work was in part supported by grants from the Instituto de Salud Carlos III and FEDER Union Europea, Una forma de hacer Europa (PI18/01231 and PI18/01582) and (PI18/01231); FEDER Union Europea, Una forma de hacer Europa (PI18/01582) and La Fe Biobank (PT17/0015/0043). Memorial Nacho Barbera´. National Institutes of Health (NIH), National Heart, Lung and Blood Institute (NHLBI: 1R01HL132401 and R01HL151737). NIH (S10 OD018135). Leducq Foundation (14 CVD 03). NIA (R21AG060413-01). The Ewing Halsell Foundation, George and Mary Josephine Hamman Foundation, and TexGen Fund from Greater Houston Community Foundation. | es |
dc.rights | info:eu-repo/semantics/embargoedAccess | es |
dc.subject | Cardiomyopathy | - |
dc.subject | Hippo pathway | - |
dc.subject | WNT pathway | - |
dc.subject | TP53 | - |
dc.subject | RNA-sequencing | - |
dc.subject | Gene expression | - |
dc.title | The EP300/TP53 pathway, a suppressor of the Hippo and canonical WNT pathways, is activated in human hearts with arrhythmogenic cardiomyopathy in the absence of overt heart failure | es |
dc.type | info:eu-repo/semantics/article | es |
dc.embargo.terms | Si | - |
dc.identifier.doi | https://doi.org/10.1093/cvr/cvab197 | - |
Aparece en las colecciones: | Artículos: Ciencias Sociosanitarias |
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CARDIOVASCULAR RESEARCH ROUDHI.pdf | 2,09 MB | Adobe PDF | ![]() Visualizar/Abrir Solicitar una copia |
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