Melatonin effects on food intake and activity rhythms in two fish species with different activity patterns : Diurnal (goldfish) and nocturnal (tench)

Abstract

Melatonin has several known physiological functions, a major one being synchronization of daily and seasonal rhythms, although melatonin has also been reported to influence food intake and behavioral rhythms with varying results depending on the species. The aim of this research was to evaluate the effects of intraperitoneal melatonin injection on food intake and locomotor activity in two different fish species: goldfish (diurnal) and tench (nocturnal), under different light regimes: constant light (LL) conditions or LD 12:12, with melatonin administration at mid-light (ML), mid-dark (MD), and after a 1-hour light pulse at MD. In addition to these acute tests, in the case of goldfish we also investigated the effects of daily melatonin administration for one week. Our results indicated that acute melatonin administration significantly decreased goldfish food intake (16 to 52 % inhibition, depending on the light regime) and locomotor activity (55 to 100 %), with the chronic treatment inducing a similar total food intake inhibition that persisted for 7 days. In tench, a nocturnal fish species, acute melatonin administration at MD and ML reduced food intake (37 and 29 %, respectively), while locomotor activity was not affected at MD and slightly increased at ML. Taken together, these results indicated that melatonin reduced food intake in both species, while its effects on locomotor activity depended on the light conditions and the activity patterns of the species.