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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Costa, Leandro | - |
dc.contributor.author | Serrano, Ignacio | - |
dc.contributor.author | Sánchez Vázquez, Francisco Javier | - |
dc.contributor.author | López Olmeda, José Fernando | - |
dc.contributor.other | Facultades, Departamentos, Servicios y Escuelas::Departamentos de la UMU::Fisiología | - |
dc.date.accessioned | 2024-02-09T11:32:18Z | - |
dc.date.available | 2024-02-09T11:32:18Z | - |
dc.date.issued | 2016 | - |
dc.identifier.citation | Journal of Comparative Physiology B 186, 775–785 (2016) | - |
dc.identifier.issn | Print: 0174-1578 | - |
dc.identifier.issn | Electrónico: 1432-136X | - |
dc.identifier.uri | http://hdl.handle.net/10201/139105 | - |
dc.description | ©<2016>. This manuscript version is made available under the CC-BY license http://creativecommons.org/licenses/ccby/4.0/ This document is the Acepted, version of a Published Work that appeared in final form in [Journal of Comparative Physiology B].To access the final edited and published work see [https://doi.org/10.1007/s00360-016-0989-x] | - |
dc.description.abstract | The present research aimed to investigate the existence of clock gene expression rhythms in tilapia, their endogenous origin, and how light and feeding cycles synchronize these rhythms. In the first experiment, two groups of fish were kept under an LD cycle and fed at two different time points: in the middle of the light (ML) or in the middle of the dark (MD) phase. In the second experiment, fish fed at ML was fasted and kept under constant lighting (LL) conditions for 1 day. In both experiments, the samples from central ( optic tectum and hypothalamus) and peripheral (liver) tissues were collected every 3 h throughout a 24 h cycle. The express10n levels of clock genes bmall a, clockl, per 1 b, cry2a, and cry5 were analyzed by quantitative PCR. All the clock genes analyzed in brain regions showed daily rhythms: clockl, bmall a, and cry2a showed the acrophase approximately at the end of the light phase (ZT 8:43-11:22 h), whereas perlb and cry5 did so between the end of the dark phase and the beginning of the light phase, respectively (ZT 21: 16-4:00 h). These rhythms persisted under constant conditions. No effect of the feeding time was observed in the brain. In the liver, however, the rhythms of clockl and cry5 were influenced by feeding, anda shift was observed in the MD fish group (ZT 3:58 h for clockl and 11 :20 h for cry5). This study provides the first insights into the molecular clock of tilapia, a very important fish species for aquaculture. It also reveals the endogenous origin of clock gene rhythms and the ability of feeding time to shift the phase in sorne clock genes in the peripheral, but not the central, oscillator. | - |
dc.format | application/pdf | es |
dc.format.extent | 27 | - |
dc.language | eng | es |
dc.publisher | Springer | - |
dc.relation | This research was funded by Project 18963/JLI/13 granted by the Agencia de Ciencia y Tecnología de la Región de Murcia (Fundacion Seneca) to JFLO and by Project AGL2013-49027-C3-1-R granted by the Spanish Ministry of Economy and Competitiveness (MINECO) to FJSV. LSC was funded through a research fellowship granted by the CAPES Foundation (Ministry of Education of Brazil) (grant number 6187/13-7). JFLO had a research contract granted by the MINECO (the Juan de la Cierva Program). | es |
dc.rights | info:eu-repo/semantics/openAccess | es |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Teleost fish | - |
dc.subject | Liver peripheral oscillator | - |
dc.subject | Hypothalamus | - |
dc.subject | Optic tectum | - |
dc.subject | Endogenous oscillations | - |
dc.subject.other | CDU::5 - Ciencias puras y naturales::57 - Ciencias biológicas en general | - |
dc.title | Circadian rhythms of clock gene expression in Nile tilapia (Oreochromis niloticus) central and peripheral tissues : Influence of different lighting and feeding conditions | es |
dc.type | info:eu-repo/semantics/article | es |
dc.identifier.doi | https://doi.org/10.1007/s00360-016-0989-x | - |
Aparece en las colecciones: | Artículos: Fisiología |
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