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https://doi.org/10.1186/s12917-022-03240-5
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Título: | Measurement of procalcitonin in saliva of pigs: a pilot study |
Fecha de publicación: | 15-abr-2022 |
Editorial: | BMC |
Cita bibliográfica: | BMC Veterinary Research 18, (2022): 139 |
ISSN: | Electronic: 1746-6148 |
Palabras clave: | Bacterial infections Biomarkers LPS Meningitis Porcine Procalcitonin Saliva Sepsis Turpentine oil |
Resumen: | Background: Procalcitonin (PCT) is a widely used biomarker of sepsis in human medicine and can have potential applications in the veterinary field. This study aimed to explore whether PCT could be measured in the saliva of pigs and whether its concentration changes in sepsis. Therefore, a specific assay was developed and analytically validated, and changes in PCT concentration were evaluated in two conditions: a) in an experimental model of sepsis produced by the administration of lipopolysaccharide (LPS) to pigs (n = 5), that was compared with a model of non‑septic inflammation induced by turpentine oil (n = 4), and b) in healthy piglets (n = 11) compared to piglets with meningitis (n = 20), a disease that usually involves sepsis and whose treatment often requires large amounts of antibiotics in farms. Results: The assay showed coefficients of variation within the recommended limits and adequate linearity after serial sample dilutions. The method’s detection limit was set at 68 μg/L, and the lower limit of quantification was 414 μg/L. In the LPS experiment, higher concentrations of PCT were found after 24 h in the animals injected with LPS (mean = 5790 μg/L) compared to those treated with turpentine oil (mean = 2127 μg/L, P = 0.045). Also, animals with meningitis had higher concentrations of PCT (mean = 21515 μg/L) than healthy pigs (mean = 6096 μg/L, P value < 0.0001). Conclusions: According to these results, this assay could be potentially used as a tool for the non‑invasive detection of sepsis in pigs, which is currently a topic of high importance due to antibiotic use restriction. |
Autor/es principal/es: | López Martínez, María Jose Escribano, Damián Martínez Miró, Silvia Ramis, Guillermo Manzanilla, Edgar G. Tecles, Fernando Martínez Subiela, Silvia Cerón, Jose J. |
Facultad/Departamentos/Servicios: | Facultades, Departamentos, Servicios y Escuelas::Departamentos de la UMU:: Medicina y Cirugía Animal |
Versión del editor: | https://bmcvetres.biomedcentral.com/articles/10.1186/s12917-022-03240-5 |
URI: | http://hdl.handle.net/10201/138947 |
DOI: | https://doi.org/10.1186/s12917-022-03240-5 |
Tipo de documento: | info:eu-repo/semantics/article |
Número páginas / Extensión: | 9 |
Derechos: | info:eu-repo/semantics/openAccess Attribution-NonCommercial-NoDerivatives 4.0 Internacional |
Descripción: | ©2022. This manuscript version is made available under the CC-BY 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ This document is the Published, version of a Published Work that appeared in final form in BMC Veterinary Research. To access the final edited and published work see https://doi.org/10.1186/s12917-022-03240-5 |
Aparece en las colecciones: | Artículos: Medicina y Cirugía Animal |
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