Por favor, use este identificador para citar o enlazar este ítem:
https://doi.org/10.1155/2019/6032141
Twittear
Título: | CD33 (Siglec-3) Inhibitory Function: Role in the NKG2D/DAP10 Activating Pathway. |
Fecha de publicación: | 2019 |
Editorial: | Hindawi |
Cita bibliográfica: | Journal of Immunology Research Volume 2019, Article ID 6032141 |
ISSN: | Print: 2314-8861 Electronic: 2314-7156 |
Materias relacionadas: | CDU::5 - Ciencias puras y naturales |
Palabras clave: | NK cells CD33 Inhibitory receptors Cytotoxicity |
Resumen: | CD33 (siglec-3), a well-known target in leukemia therapy, is an inhibitory sialoadhesin expressed in human leukocytes of the myeloid lineage and some lymphoid subsets, including NK cells. It may constitute a control mechanism of the innate immune system; nevertheless, its role as an inhibitory receptor remains elusive. Using human NK cells as a cellular model, we analyzed CD33 inhibitory function upon different activating receptors. In high-cytotoxicity NKL cells, CD33 displayed a prominent inhibition on cytotoxicity triggered by the activating receptors NKG2D and, in a lower extent, 2B4, whereas it did not inhibit NKp46-induced cytotoxicity. NKp46 was partially inhibited by CD33 only when low-cytotoxicity NKL cells were tested. CD33 triggering did not inhibit IFN-γ secretion, contrasting with ILT-2 and CD94/NKG2A inhibitory receptors that inhibited cytotoxicity and IFN-γ secretion induced by all activating receptors tested. CD33-mediated inhibition of NKG2D-induced triggering involved Vav1 dephosphorylation. Our results support the role of CD33 as an inhibitory receptor preferentially regulating the NKG2D/DAP10 cytotoxic signaling pathway, which could be involved in self-tolerance and tumor and infected cell recognition. |
Autor/es principal/es: | Hernández-Caselles, Trinidad Corral-San Miguel, Rubén Ruiz-Alcaraz, Antonio José García-Peñarrubia, Pilar |
Facultad/Departamentos/Servicios: | Facultades, Departamentos, Servicios y Escuelas::Departamentos de la UMU::Bioquímica y Biología Molecular "B" e Inmunología |
URI: | http://hdl.handle.net/10201/138943 |
DOI: | https://doi.org/10.1155/2019/6032141 |
Tipo de documento: | info:eu-repo/semantics/article |
Número páginas / Extensión: | 15 |
Derechos: | info:eu-repo/semantics/openAccess |
Descripción: | Journal of Immunology Research is an open access journal. All articles are immediately available to read and reuse upon publication. More information about our Open Access policy can be found on our copyright page https://www.hindawi.com/copyright/. |
Aparece en las colecciones: | Artículos: Bioquímica y Biología Molecular "B" e Inmunología |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
JIR2019-6032141.pdf | 1,89 MB | Adobe PDF | Visualizar/Abrir |
Los ítems de Digitum están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.