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https://doi.org/10.1016/j.exer.2023.109627
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Título: | Neuroinflammation and gliosis in the injured and contralateral retinas after unilateral optic nerve crush |
Fecha de publicación: | 22-ago-2023 |
Editorial: | Elsevier |
Cita bibliográfica: | Experimental Eye Research, 235 (2023): 109627 |
ISSN: | Print: 0014-4835 |
Palabras clave: | Oftalmología Neurociencia Inflamación Retina Optic nerve crush Inflammation Cytokines Gliosis Microglial cells Bilateral response |
Resumen: | The main purpose of this study is to analyze the effects of unilateral optic nerve crush in the gene expression of pro- and anti-inflammatory mediators, and gliosis markers in injured and contralateral retinas. Retinas from intact, unilaterally optic nerve injured or sham-operated C57BL/6J mice were analyzed 1, 3, 9 and 30 days after the surgery (n = 5/group and time point) and the relative expression of TGF-β1, IL-1β, TNF-α, Iba1, AQP4, GFAP, MHCII, and TSPO was analyzed in injured and contralateral using qPCR. The results indicated that compared with intact retinas, sham-operated animals showed an early (day 1) upregulation of IL-1β, TNF-α and TSPO and a late (day 30) upregulation of TNF-α. In sham-contralateral retinas, TNF-α and TSPO mRNA expression were upregulated and day 30 while GFAP, Iba1, AQP4 and MHCII downregulated at day 9. Compared with sham-operated animals, in retinas affected by optic nerve crush GFAP and TSPO upregulated at day 1 and TNF-α, Iba1, AQP4 and MHCII at day 3. In the crushed-contralateral retinas, TGF-β1, TNF-α, Iba1 and MHCII were upregulated at day 1. TSPO was upregulated up to day 30 whereas TGF-β1 and Iba1 downregulated after day 9. In conclusion, both sham surgery and optic nerve crush changed the profile of inflammatory and gliosis markers in the injured and contralateral retinas, changes that were more pronounced for optic nerve crush when compared to sham. |
Autor/es principal/es: | Cabrera Maqueda, José María Boia, Raquel Lucas Ruiz, Fernando González Riquelme, María José Ambrosio, Antonio Francisco Santiago, Ana Raquel Vidal Sanz, Manuel Agudo Barriuso, Marta Galindo Romero, Caridad |
Facultad/Departamentos/Servicios: | Facultades, Departamentos, Servicios y Escuelas::Departamentos de la UMU::Oftalmología |
URI: | http://hdl.handle.net/10201/138882 |
DOI: | https://doi.org/10.1016/j.exer.2023.109627 |
Tipo de documento: | info:eu-repo/semantics/article |
Número páginas / Extensión: | 6 |
Derechos: | info:eu-repo/semantics/embargoedAccess Attribution-NonCommercial-NoDerivatives 4.0 Internacional |
Descripción: | ©2023. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ This document is the Accepted, version of a Published Work that appeared in final form in Experimental Eye Research. To access the final edited and published work see https://doi.org/10.1016/j.exer.2023.109627 |
Aparece en las colecciones: | Artículos: Oftalmología, Optometría, Otorrinolaringología y Anatomía Patológica |
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35_Cabrera-Maqueda.pdf | 2,69 MB | Adobe PDF | Visualizar/Abrir Solicitar una copia |
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