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https://doi.org/10.1016/j.theriogenology.2019.08.010
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Título: | Levels of activity of superoxide dismutase in seminal plasma do not predict fertility of pig AI-semen doses |
Fecha de publicación: | dic-2019 |
Cita bibliográfica: | Theriogenology (2019) 140:18-24. |
ISSN: | 0093-691X 1879-3231 |
Palabras clave: | Superoxide dismutase Artificial Insemination Fertility Seminal Plasma Pig |
Resumen: | Superoxide dismutase (SOD) is a major antioxidant enzyme in boar seminal plasma (SP). This study evaluated how SP-SOD affected sperm attributes when semen of boars of various breeds, included in commercial artificial insemination (AI)-programs, was extended and liquid-stored at 17 °C for AI; as well as their in vivo fertility (farrowing rate and litter size of 10,952 AI-sows). SP-SOD-activity was assessed in 311 ejaculates (100 boars) while sperm motility (by CASA), viability and intracellular H2O2 generation in viable spermatozoa (by flow cytometry) were measured at 0 and 72 h of liquid storage. SP-SOD activity was not affected by breed but differed (P < 0.001) between boars (n = 50), ranging from 1.16 ± 0.11 to 7.02 ± 0.75 IU/mL. Semen AI-doses (n = 44) hierarchically grouped (P < 0.001) with low SP-SOD activity showed lower (P < 0.05) sperm motility and intracellular H2O2 at 72 h of liquid storage. Fertility did not differ between AI-boars (n = 39) hierarchically grouped (P < 0.001) with high or low SP-SOD activity. In conclusion, SP-SOD activity is boar dependent and positively related with sperm functionality of liquid-stored semen AI-doses. However, this positive effect is not reflected on in vivo fertility post-AI. |
Autor/es principal/es: | Barranco, Isabel Padilla, Lorena Tvarijonaviciute, Asta Parrilla, Inmaculada Martinez, Emilio A. Rodríguez Martínez, Heriberto Yeste, Marc Roca, Jordi |
Facultad/Departamentos/Servicios: | Facultades, Departamentos, Servicios y Escuelas::Departamentos de la UMU::Medicina y Cirugía Animal Facultades, Departamentos, Servicios y Escuelas::Facultades de la UMU::Facultad de Veterinaria |
Versión del editor: | https://www.sciencedirect.com/science/article/pii/S0093691X19303504?via%3Dihub |
URI: | http://hdl.handle.net/10201/138671 |
DOI: | https://doi.org/10.1016/j.theriogenology.2019.08.010 |
Tipo de documento: | info:eu-repo/semantics/article |
Derechos: | info:eu-repo/semantics/openAccess Attribution-NonCommercial-NoDerivatives 4.0 Internacional |
Descripción: | ©2019. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ This document is the Accepted, version of a Published Work that appeared in final form in Theriogenology. To access the final edited and published work see https://doi.org/10.1016/j.theriogenology.2019.08.010 |
Aparece en las colecciones: | Artículos: Medicina y Cirugía Animal |
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