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https://doi.org/10.3389/fvets.2019.00436
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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Barranco, Isabel | - |
dc.contributor.author | Padilla, Lorena | - |
dc.contributor.author | Perez-Patiño, Cristina | - |
dc.contributor.author | Vazquez, Juan M | - |
dc.contributor.author | Martinez, Emilio A | - |
dc.contributor.author | Rodriguez-Martinez, Heriberto | - |
dc.contributor.author | Roca, Jordi | - |
dc.contributor.author | Parrilla, Inmaculada | - |
dc.contributor.other | Facultades, Departamentos, Servicios y Escuelas::Facultades de la UMU::Facultad de Veterinaria | es |
dc.contributor.other | Facultades, Departamentos, Servicios y Escuelas::Departamentos de la UMU::Medicina y Cirugía Animal | es |
dc.date.accessioned | 2024-02-02T12:52:14Z | - |
dc.date.available | 2024-02-02T12:52:14Z | - |
dc.date.issued | 2019-12-04 | - |
dc.identifier.citation | Frontiers in Veterinary Science 2019. 6:436. | es |
dc.identifier.uri | http://hdl.handle.net/10201/138551 | - |
dc.description | ©2019. This manuscript version is made available under the CC-BY 4.0 license http://creativecommons.org/licenses/by /4.0/ This document is the Published, version of a Published Work that appeared in final form in Frontiers in Veterinary Science. To access the final edited and published work see https://doi.org/10.3389/fvets.2019.00436 | - |
dc.description.abstract | Background: Boar seminal plasma is rich in cytokines, which could influence the capability of spermatozoa to tolerate preservation. Objectives: To evaluate the involvement of boar seminal plasma cytokines in the changes experienced by boar spermatozoa during their storage, either in liquid or frozen state. Materials and Methods: In two separated experiments, semen samples from healthy and fertile boars were split in two aliquots, one centrifuged twice (1,500 ×g for 10 min) to harvest seminal plasma, whereas the other was either commercially extended (3 × 107 sperm/mL) and liquid-stored at 17°C during 144 h (n = 28, Experiment 1) or frozen-thawed using a standard 0.5 mL protocol (n = 27, Experiment 2). Sixteen cytokines were quantified using Luminex xMAP®. Sperm attributes (CASA-evaluated total and progressive motility; flow cytometry-evaluated sperm viability, production of intracellular H2O2 and O∙−2 and levels of lipid peroxidation in viable spermatozoa) were evaluated either at 0, 72, or 144 h of liquid storage (Experiment 1) or before freezing and at 30- and 150-min post-thawing (Experiment 2). Results: Multiple linear regression models, with Bayesian approach for variable selection, revealed that the anti-inflammatory TGF-β2, TGF-β3, IL-1Ra, and IL-4 and the pro-inflammatory IL-8 and IL-18, predicted changes in sperm motility for liquid-stored semen while the anti-inflammatory IFN-γ was included in the models predicting changes in all sperm attributes for cryopreserved semen. Conclusion: Specific boar seminal plasma cytokines would contribute to modulate the structural and metabolic changes shown by spermatozoa during preservation, either in liquid or frozen state. | es |
dc.format | application/pdf | es |
dc.format.extent | 10 | - |
dc.language | eng | es |
dc.relation | MINECO and FEDER [AGL2015-69738-R] Madrid (Spain), Seneca Foundation [19892/GERM/15] Murcia (Spain), Swedish Research Council Formas [2017-00946], Swedish Research Council [Vetenskapsrådet, VR, 2015-05919], and FORSS [745971, Stockholm, Sweden] | es |
dc.rights | info:eu-repo/semantics/openAccess | es |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights | Atribución 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject | Seminal plasma | es |
dc.subject | Cytokines | - |
dc.subject | Spermatozoa | - |
dc.subject | Liquid storage | - |
dc.subject | Cryopreservation | - |
dc.subject | Pig | - |
dc.title | Seminal plasma cytokines are predictive of the outcome of boar sperm preservation | es |
dc.type | info:eu-repo/semantics/article | es |
dc.relation.publisherversion | https://www.frontiersin.org/articles/10.3389/fvets.2019.00436/full | es |
dc.identifier.doi | https://doi.org/10.3389/fvets.2019.00436 | - |
Aparece en las colecciones: | Artículos: Medicina y Cirugía Animal |
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