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10.3390/ijms20071791
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Título: | Cryopreservation differentially alters the proteome of epididymal and ejaculated pig spermatozoa |
Fecha de publicación: | 11-abr-2019 |
Cita bibliográfica: | International Journal of Molecular Sciences. 20(7):1791. |
ISSN: | 1661-6596 1422-0067 |
Palabras clave: | cryopreservation; ejaculate; epididymis; porcine; proteomics; spermatozoa |
Resumen: | Cryopreservation induces differential remodeling of the proteome in mammalian spermatozoa. How these proteome changes relate to the loss of sperm function during cryopreservation remains unsolved. The present study aimed to clarify this issue evaluating differential changes in the proteome of fresh and frozen-thawed pig spermatozoa retrieved from the cauda epididymis and the ejaculate of the same boars, with clear differences in cryotolerance. Spermatozoa were collected from 10 healthy, sexually mature, and fertile boars, and cryopreserved using a standard 0.5 mL-straw protocol. Total and progressive motility, viability, and mitochondria membrane potential were higher and membrane fluidity and reactive oxygen species generation lower in frozen-thawed (FT) epididymal than ejaculated spermatozoa. Quantitative proteomics of fresh and FT spermatozoa were analyzed using a LC-ESI-MS/MS-based Sequential Window Acquisition of All Theoretical Spectra approach. Cryopreservation quantitatively altered more proteins in ejaculated than cauda epididymal spermatozoa. Differential protein-protein networks highlighted a set of proteins quantitatively altered in ejaculated spermatozoa, directly involved in mitochondrial functionality which would explain why ejaculated spermatozoa deteriorate during cryopreservation. |
Autor/es principal/es: | Perez-Patiño, Cristina Barranco, Cascales Li, Junwei Padilla, Lorena Martínez, Emilio A Rodriguez-Martinez, Heriberto Roca, Jordi Parrilla, Inmaculada |
Facultad/Departamentos/Servicios: | Facultades, Departamentos, Servicios y Escuelas::Departamentos de la UMU::Medicina y Cirugía Animal |
Versión del editor: | https://www.mdpi.com/1422-0067/20/7/1791 |
URI: | http://hdl.handle.net/10201/138524 |
DOI: | 10.3390/ijms20071791 |
Tipo de documento: | info:eu-repo/semantics/article |
Derechos: | info:eu-repo/semantics/openAccess Attribution-NonCommercial-NoDerivatives 4.0 Internacional |
Descripción: | Item en revisión. Pendiente de cumplimentar metadatos. |
Aparece en las colecciones: | Artículos: Medicina y Cirugía Animal |
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