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10.1007/bf03320822
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Título: | Pulp cell cultures obtained with two different methods for in vitro cytotoxicity tests. |
Fecha de publicación: | 2006 |
Cita bibliográfica: | European Archives of Paediatric Dentistry. 2006, 7, 96-99. ISSN: 19969805 |
ISSN: | 1996-9805 1818-6300 |
Palabras clave: | Pulp cell cultures Cytotoxicity |
Resumen: | Aim: To describe two different protocols for obtaining pri- mary pulp cell cultures, one derived from explants and the other following dissociation into single cell suspension by enzyme digestion. Methods: Human pulp tissue was obtained from three healthy premolars. The harvested pulp tissue was prepared for culture using physical methods (one of the premolars) and enzyme, type XI collagenase, (the two remaining premolars). Results: In the case of explant based culture, only limited growth was observed in some cases. However, by enzyme digestion, after two weeks cell growth was evident, and differences in cell type were observed according to the tooth involved. Conclusion: It has been possible to obtain abundant biological material using an enzyme digestion-based protocol for testing purposes, with low experimental variability, as all cells originated from the same individual. |
Autor/es principal/es: | Cortés, Olga García, Carlos Pérez, Leonor Boj, Juan R. Alcaina, Antonia |
Facultad/Departamentos/Servicios: | Facultades, Departamentos, Servicios y Escuelas::Departamentos de la UMU::Dermatología, Estomatología, Radiología y Medicina Física |
URI: | http://hdl.handle.net/10201/138417 |
DOI: | 10.1007/bf03320822 |
Tipo de documento: | info:eu-repo/semantics/article |
Número páginas / Extensión: | 4 |
Derechos: | info:eu-repo/semanticsembargoedAccess |
Descripción: | Se puede depositar en abierto la versión aceptada |
Aparece en las colecciones: | Artículos: Dermatología, Estomatología, Radiología y Medicina Física |
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Cortés_Article_PulpCellCulturesObtainedWithTw.pdf | 260,47 kB | Adobe PDF | Visualizar/Abrir Solicitar una copia |
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