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https://doi.org/10.1016/j.dental.2022.06.019
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Título: | Bone regeneration in critical-sized mandibular symphysis defects using bioceramics with or without bone marrow mesenchymal stem cells in healthy, diabetic, osteoporotic, and diabetic-osteoporotic rats |
Fecha de publicación: | 2022 |
Editorial: | Elsevier SCI LTD |
ISSN: | 0109-5641 |
Materias relacionadas: | CDU::5 - Ciencias puras y naturales |
Palabras clave: | Bioceramics Bone marrow mesenchymal stem cells Mandibular symphysis Diabetes Osteoporosis |
Resumen: | Objectives: To compare new bone formation in mandibular critical-sized bone defects (CSBDs) in healthy, diabetic, osteoporotic, and diabetic-osteoporotic rats filled with bioceramics (BCs) with or without bone marrow mesenchymal stem cells (BMSCs). Methods: A total of 64 adult female Sprague-Dawley rats were randomized into four groups (n = 16 per group): Group 1 healthy, Group 2 diabetic, Group 3 osteoporotic, and Group 4 diabetic-osteoporotic rats. Streptozotocin was used to induce type 1 diabetes in Group 2 and 4, while bilateral ovariectomy was used to induce osteoporosis in Group 3 and 4. The central portion of the rat mandibular symphysis was used as a physiological CSBD. In each group, eight defects were filled with BC (hydroxypatatite 60% and β-tricalcium phosphate 40%) alone and eight with BMSCs cultured on BC. The animals were sacrificed at 4 and 8 weeks, and the mandibles were processed for micro-computed tomography to analyze radiological union and bone mineral density (BMD); histological analysis of the bone union; and immunohistochemical analysis, which included immunoreactivity of vascular endothelial growth factor (VEGF) and bone morphogenetic protein 2 (BMP-2). Results: In all groups (healthy, diabetics, osteoporotics, and diabetics-osteoporotics), the CSBDs filled with BC + BMSCs showed greater radiological bone union, BMD, histological bone union, and more VEGF and BMP-2 positivity, in comparison with CSBDs treated with BC alone (at 4 and 8 weeks). |
Autor/es principal/es: | Camacho Alonso, Fabio Tudela Mulero, M.R. Buendía, A.J. Navarro, J.A. Pérez-Sayáns, M. Mercado Díaz, A.M. |
Facultad/Departamentos/Servicios: | Facultades, Departamentos, Servicios y Escuelas::Departamentos de la UMU::Dermatología, Estomatología, Radiología y Medicina Física |
URI: | http://hdl.handle.net/10201/138326 |
DOI: | https://doi.org/10.1016/j.dental.2022.06.019 |
Tipo de documento: | info:eu-repo/semantics/article |
Número páginas / Extensión: | 18 |
Derechos: | info:eu-repo/semantics/embargoedAccess |
Aparece en las colecciones: | Artículos: Dermatología, Estomatología, Radiología y Medicina Física |
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Fichero | Descripción | Tamaño | Formato | |
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1-s2.0-S0109564122001592-main.2022B (33).pdf | 13,9 MB | Adobe PDF | Visualizar/Abrir Solicitar una copia |
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