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https://doi.org/10.1002/JPER.21-0306
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Título: | Synergistic antimicrobial effect of photodynamic therapy and chitosan on the titanium-adherent biofilms of Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa: An in vitro study |
Fecha de publicación: | 2022 |
Editorial: | Wiley |
Cita bibliográfica: | Journal of Periodontology 2022; 93(6):e104-e115 |
ISSN: | 0022-3492 |
Materias relacionadas: | CDU::5 - Ciencias puras y naturales |
Palabras clave: | Chitosan Escherichia coli Phototherapy Pseudomonas aeruginosa Staphylococcus aureus |
Resumen: | Background: To date, no studies on the combined use of photodynamic therapy (PDT) and chitosan against peri-implantitis have been published. The aim of this study was to evaluate the possible synergistic antimicrobial effect of PDT and chitosan on the titanium-adherent biofilms of Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa. Methods: A total of 60 titanium discs were included in this study. The discs were randomized into three bacterial contaminations (n = 20 discs per bacterium). After being cultured (incubated for 48 hours) they were randomized again into four different disinfection modalities (n = 5 discs per treatment): control (without treatment), PDT, chitosan 3 mg/mL, and PDT + chitosan 3 mg/mL. After the treatments, the colony forming units (CFU) were measured to determine antimicrobial effects, and field emission scanning electron microscopy (FESEM) was used to study cell morphology and titanium adherence. Results: For all the evaluated bacteria and all the variables studied the order from highest to lowest antimicrobial effectiveness was: PDT + chitosan 3 mg/mL > chitosan 3 mg/mL > PDT > control. Although, all disinfection methods were significantly effective when compared to control, the combined treatment of PDT + chitosan 3 mg/mL had the greatest antimicrobial effect against the three studied bacteria. Conclusions: The combination of PDT and chitosan has a synergistic antimicrobial effect against the bacteria S. aureus, E. coli, and P. aeruginosa, all closely related to peri-implantitis. However, further in vivo studies are needed because this study provides data based on an in vitro scenario that might not be extrapolated to patients with peri-implantitis. |
Autor/es principal/es: | Camacho Alonso, Fabio Salinas, Jesús Sánchez Siles, Mariano Pato Mourelo, Jesús Cotrina Veizaga, Brian Davis Ortega, Nieves |
Facultad/Departamentos/Servicios: | Facultades, Departamentos, Servicios y Escuelas::Departamentos de la UMU::Dermatología, Estomatología, Radiología y Medicina Física |
URI: | http://hdl.handle.net/10201/138325 |
DOI: | https://doi.org/10.1002/JPER.21-0306 |
Tipo de documento: | info:eu-repo/semantics/article |
Número páginas / Extensión: | 12 |
Derechos: | info:eu-repo/semantics/embargoedAccess |
Aparece en las colecciones: | Artículos: Dermatología, Estomatología, Radiología y Medicina Física |
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Journal of Periodontology - 2021 - Camacho‐Alonso - Synergistic antimicrobial effect of photodynamic therapy and chitosan.2022A (30).pdf | 1,54 MB | Adobe PDF | Visualizar/Abrir Solicitar una copia |
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