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https://doi.org/10.1016/j.cellsig.2007.12.017
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Título: | Activation of the cell integrity pathway is channelled through diverse signalling elements in fission yeast |
Fecha de publicación: | 4-ene-2008 |
Editorial: | ELSEVIER |
Cita bibliográfica: | Cellular Signalling Vol. 20(4):748-757. 2008 |
ISSN: | Print: 0898-6568 |
Materias relacionadas: | CDU::5 - Ciencias puras y naturales::57 - Biología::579 - Microbiología CDU::5 - Ciencias puras y naturales::57 - Biología::576 - Biología celular y subcelular. Citología CDU::5 - Ciencias puras y naturales::57 - Biología::577 - Bioquímica. Biología molecular. Biofísica |
Palabras clave: | Microbiology Fission yeast MAP kinase Cell signalling Cell integrity pathgway |
Resumen: | MAPK Pmk1p is the central element of a cascade involved in the maintenance of cell integrity and other functions in Schizosaccharomyces pombe. Pmk1p becomes activated by multiple stressing situations and also during cell separation. GTPase Rho2p acts upstream of the protein kinase C homolog Pck2p to activate the Pmk1 signalling pathway through direct interaction with MAPKKK Mkh1p. In this work we analyzed the functional significance of both Rho2p and Pck2p in the transduction of various stress signals by the cell integrity pathway. The results indicate that basal Pmk1p activity can be positively regulated by alternative mechanisms which are independent on the control by Rho2p and/or Pck2p. Unexpectedly, Pck1p, another protein kinase C homolog, negatively modulates Pmk1p basal activity by an unknown mechanism. Moreover, different elements appear to regulate the stress-induced activation of Pmk1p depending on the nature of the triggering stimuli. Whereas Pmk1p activation induced by hyper- or hypotonic stresses is channeled through Rho2p–Pck2p, other stressors, like glucose deprivation or cell wall disturbance, are transduced via other pathways in addition to that of Rho2p–Pck2p. On the contrary, Pmk1p activation observed during cell separation or after treatment with hydrogen peroxide does not involve Rho2p–Pck2p. Finally, Pck2p function is critical to maintain a Pmk1p basal activity that allows Pmk1p activation induced by heat stress. These data demonstrate the existence of a complex signalling network modulating Pmk1p activation in response to a variety of stresses in fission yeast. |
Autor/es principal/es: | Barba, Gregorio Soto, Teresa Madrid, Marisa Núñez, Andrés Vicente, Jero Gacto, Mariano Cansado, José |
Facultad/Departamentos/Servicios: | Facultades, Departamentos, Servicios y Escuelas::Departamentos de la UMU::Genética y Microbiología |
Versión del editor: | https://www.sciencedirect.com/science/article/pii/S0898656807003944 |
URI: | http://hdl.handle.net/10201/138235 |
DOI: | https://doi.org/10.1016/j.cellsig.2007.12.017 |
Tipo de documento: | info:eu-repo/semantics/article |
Número páginas / Extensión: | 10 |
Derechos: | info:eu-repo/semantics/embargoedAccess |
Descripción: | © 2008 Elsevier Inc. All rights reserved. This document is the Published version of a Published Work that appeared in final form in Cellular Signalling. To access the final edited and published work see https://doi.org/10.1016/j.cellsig.2007.12.017 |
Aparece en las colecciones: | Artículos: Genética y Microbiología |
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