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Título: | Influence of the way of reporting alpha- Amylase values in saliva in different naturalistic situations: A pilot study |
Fecha de publicación: | 27-jun-2017 |
Editorial: | Ruud van den Bos, Radboud Universiteit, NETHERLANDS Received: |
Cita bibliográfica: | Plos One, 12(6), 2017 |
ISSN: | 1932-6203 |
Palabras clave: | Saliva alpha-amylase Human Stress |
Resumen: | The objective of this pilot study was to compare the different ways of measuring salivary alpha-amylase (sAA, enzymatic vs. concentration) and to evaluate the influence that the dif- ferent ways of reporting the results can have in sAA interpretation. For this purpose, sAA was measured by direct quantification and also by an enzymatic assay in three different nat- uralistic situations, a physical stressor (situation 1) and two mental stressors of different intensity (situations 2 and 3). The results were expressed in three different ways (without correction, multiplied by flow rate and divided by protein concentration). sAA concentration and activity increased just after situations 1 and 3. When values were multiplied by the flow rate, significant changes after situation 1 were detected only for sAA activity but not for sAA concentration, being these changes of lower significance and magnitude that those observed for sAA activity without any correction. In addition, a significant increase in sAA activity was found at T+15 in situation 2. In situation 3 the significant decrease in sAA at T+15 disappeared. When values were divided by protein concentration, there were no sig- nificant changes in situations 1 or 3, but a decrease in situation 2 at T+0 and an increase at T+15. sAA activity and concentration showed a significant correlation in all situations. This pilot study points out that the way of expressing sAA can influence the results obtained in dif- ferent stress models and also their interpretation. Therefore, how sAA is reported and the factors involved in the different ways of expressing sAA, should be taken into consideration for an objective interpretation of sAA values. |
Autor/es principal/es: | Contreras Aguilar, María Dolores Escribano Tortosa, Damián Martínez Subiela, Silvia Martínez Miró, Silvia Rubio, Mónica Tvarijonaviciute, Asta Tecles Vicente, Fernando Cerón Madrigal, José Joaquín |
Facultad/Departamentos/Servicios: | Facultades, Departamentos, Servicios y Escuelas::Departamentos de la UMU::Medicina y Cirugía Animal Facultades, Departamentos, Servicios y Escuelas::Departamentos de la UMU::Producción Animal Department of Medicine and Animal Surgey, Veterinary school, Universitat CEU Cardenal Herrera, Valencia, Spain |
Versión del editor: | https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0180100 |
URI: | http://hdl.handle.net/10201/138116 |
DOI: | https://doi.org/10.1371/ journal.pone.0180100 |
Tipo de documento: | info:eu-repo/semantics/article |
Número páginas / Extensión: | 13 |
Derechos: | info:eu-repo/semantics/openAccess Attribution-NonCommercial-NoDerivatives 4.0 Internacional |
Descripción: | ©2017. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ This document is the Published, version of a Published Work that appeared in final form in Plos ONE. To access the final edited and published work see https://doi.org/10.1371/ journal.pone.0180100 |
Aparece en las colecciones: | Artículos: Medicina y Cirugía Animal |
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Contreras-Aguilar et al. - 2017 - Influence of the way of reporting alpha- Amylase values in saliva in different naturalistic situations.pdf | 1,88 MB | Adobe PDF | Visualizar/Abrir |
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