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https://doi.org/10.1016/j.prevetmed.2023.106075
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Título: | Serological and molecular survey of canine distemper virus in red foxes (Vulpes vulpes): Exploring cut-off values and the use of protein A in ELISA tests |
Fecha de publicación: | dic-2023 |
Editorial: | Elsevier GmbH |
Cita bibliográfica: | Preventive Veterinary Medicine 221,106075, 2023 |
ISSN: | Print: 0167-5877 Electronic: 1873-1716 |
Materias relacionadas: | CDU::5 - Ciencias puras y naturales::57 - Biología::574 - Ecología general y biodiversidad CDU::5 - Ciencias puras y naturales::57 - Biología::579 - Microbiología CDU::6 - Ciencias aplicadas::63 - Agricultura. Silvicultura. Zootecnia. Caza. Pesca::636 - Veterinaria. Explotación y cría de animales. Cría del ganado y de animales domésticos |
Palabras clave: | Protein A ELISA Vulpes vulpes Morbillivirus Wildlife |
Resumen: | The wide distribution and ecological plasticity of the red fox (Vulpes vulpes) make it a potential reservoir for many infectious diseases shared with domestic and wild carnivores. One of such diseases is canine distemper, which is caused by an RNA virus and its main domestic reservoir is the dog. However, other carnivores can also participate in its maintenance, as shown by the recent upsurge of reported cases in wildlife in many parts of the world, and by the fact that red foxes may act as true reservoirs for canine distemper virus (CDV). The lack of validated serological tests for wildlife or other non-target species may be a handicap for monitoring this virus. In this study, serological assays were compared in 147 red fox sera using a commercial ELISA validated for its use in dogs and a non-specific modified ELISA with Protein A peroxidase conjugate to detect bound antibodies. In addition, the presence of CDV RNA in brain, spleen, lung, and liver samples from 144 foxes was investigated by a RT-qPCR. Through the comparison of the results of both ELISAs and the use of a finite mixture model of the optical density values obtained by both techniques, we adjusted the cut-off point of the commercial ELISA to obtain the seroprevalence in foxes. The overall seroprevalence detected was 53.7% (79/147) and 57.1% (84/147) by the commercial and modified ELISA, respectively, with a moderate agreement according to Cohen’s Kappa statistic (κ = 0.491, z = 5.97, p < 0.0001). CDV RNA was detected in 30 out of 144 foxes, which resulted in 20.8% of CDV-infected foxes. At individual level, the results obtained by relating the serological status and the presence/absence of RNA in different organs were explained in terms of the pathogenesis of the infection. Our results highlight the convenience of adjusting the cut-off point when using an ELISA assay developed in domestic dogs for its use in foxes. Moreover, Protein A is confirmed to be a good alternative to be used in red foxes, presenting a good reactivity towards its IgG. |
Autor/es principal/es: | Munoz-Hernandez, C. Wipf, A. Ortega, N. Barbera, G.G. Salinas, J. Gonzalvez, M. Martinez-Carrasco, C. Candela, M. G. |
Facultad/Departamentos/Servicios: | Departamento de Sanidad Animal, Facultad de Veterinaria, Campus de Excelencia Internacional Regional “Campus Mare Nostrum”, Universidad de Murcia, 30100 Murcia, Spain Grupo Sanidad y Biotecnología (SaBio), Instituto de Investigaci´on en Recursos Cineg´eticos, IREC (CSIC-UCLM-JCCM), 13005 Ciudad Real, Spain Department of Water and Soil Conservation, CEBAS-CSIC, Campus Universitario, Espinardo 30100, Spain Departamento de Sanidad Animal, Grupo de Investigacion en Sanidad Animal y Zoonosis (GISAZ), Universidad de Cordoba, 14014 Cordoba, Spain |
URI: | http://hdl.handle.net/10201/138082 |
DOI: | https://doi.org/10.1016/j.prevetmed.2023.106075 |
Tipo de documento: | info:eu-repo/semantics/article |
Número páginas / Extensión: | 8 |
Derechos: | info:eu-repo/semantics/embargoedAccess |
Aparece en las colecciones: | Artículos: Sanidad Animal |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
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Muñoz-Hernandez et al., 2023_PVM_CDV serology.pdf | 1,3 MB | Adobe PDF | Visualizar/Abrir Solicitar una copia |
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