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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Madrid, Marisa | - |
dc.contributor.author | Núñez, Andrés | - |
dc.contributor.author | Soto, Teresa | - |
dc.contributor.author | Vicente, Jero | - |
dc.contributor.author | Gacto, Mariano | - |
dc.contributor.author | Cansado, José | - |
dc.contributor.other | Facultades, Departamentos, Servicios y Escuelas::Departamentos de la UMU::Genética y Microbiología | es |
dc.date.accessioned | 2024-01-29T11:34:12Z | - |
dc.date.available | 2024-01-29T11:34:12Z | - |
dc.date.issued | 2007-08-29 | - |
dc.identifier.citation | Molecular Biology of the Cell Vol 18(11):4405– 441. 2007 | es |
dc.identifier.issn | 1939-4586 | - |
dc.identifier.issn | 1059-1524 | - |
dc.identifier.uri | http://hdl.handle.net/10201/137934 | - |
dc.description | ©<2007>. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ This document is the Published Manuscript version of a Published Work that appeared in final form in [Molecular Biology of the Cell]. To access the final edited and published work see [https://doi.org/10.1091/mbc.e07-05-0484] | es |
dc.description.abstract | Fission yeast mitogen-activated protein kinase (MAPK) Pmk1p is involved in morphogenesis, cytokinesis, and ion homeostasis as part of the cell integrity pathway, and it becomes activated under multiple stresses, including hyper- or hypotonic conditions, glucose deprivation, cell wall-damaging compounds, and oxidative stress. The only protein phosphatase known to dephosphorylate and inactivate Pmk1p is Pmp1p. We show here that the stress-activated protein kinase (SAPK) pathway and its main effector, Sty1p MAPK, are essential for proper deactivation of Pmk1p under hypertonic stress in a process regulated by Atf1p transcription factor. We demonstrate that tyrosine phosphatases Pyp1p and Pyp2p, and serine/threonine phosphatase Ptc1p, that negatively regulate Sty1p activity and whose expression is dependent on Sty1p-Atf1p function, are involved in Pmk1p dephosphorylation under osmostress. Pyp1p and Ptc1p, in addition to Pmp1p, also control the basal level of MAPK Pmk1p activity in growing cells and associate with, and dephosphorylate Pmk1p both in vitro and in vivo. Our results with Ptc1p provide the first biochemical evidence for a PP2C-type phosphatase acting on more than one MAPK in yeast cells. Importantly, the SAPK-dependent down-regulation of Pmk1p through Pyp1p, Pyp2p, and Ptc1p was not complete, and Pyp1p and Ptc1p phosphatases are able to negatively regulate MAPK Pmk1p activity by an alternative regulatory mechanism. Our data also indicate that Pmk1p phosphorylation oscillates as a function of the cell cycle, peaking at cell separation during cytokinesis, and that Pmp1p phosphatase plays a main role in regulating this process. | es |
dc.format | application/pdf | es |
dc.format.extent | 15 | es |
dc.language | eng | es |
dc.publisher | American Society for Cell Biology | es |
dc.relation | Ámbito del proyecto: Nacional. Agencia financiadora: Ministerio de Educación y Ciencia (MEC). Convocatoria.2005. Nombre del proyecto: Dianas celulares y mecanismos de control del crecimiento regulados por la MAPK Spm1 en S. pombe. Caracterización de los circuitos moleculares que definen su activación Código o número del acuerdo de subvención: BFU2005-01401/BMC. Ámbito del proyecto: Regional. Agencia financiadora: Fundación Séneca (Región de Murcia). Convocatoria:2004. Nombre del proyecto: Estudio sobre la activación por estrés de la ruta de MAP quinasas de integridad celular en Schizosaccharomyces pombe. Código o número del acuerdo de subvención: 00475/PI/04. | es |
dc.rights | info:eu-repo/semantics/openAccess | es |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | MAP kinase | es |
dc.subject | Cell signalling | es |
dc.subject | fission yeast | es |
dc.subject | microbiology | es |
dc.subject | protein phosphatase | es |
dc.subject.other | CDU::5 - Ciencias puras y naturales::57 - Biología::579 - Microbiología | es |
dc.subject.other | CDU::5 - Ciencias puras y naturales::57 - Biología::576 - Biología celular y subcelular. Citología | es |
dc.subject.other | CDU::5 - Ciencias puras y naturales::57 - Biología::577 - Bioquímica. Biología molecular. Biofísica | es |
dc.title | Stress-activated protein kinase-mediated down-regulation of the cell integrity pathway mitogen-activated protein kinase Pmk1p by protein phosphatases | es |
dc.type | info:eu-repo/semantics/article | es |
dc.relation.publisherversion | https://www.molbiolcell.org/doi/full/10.1091/mbc.e07-05-0484 | es |
dc.identifier.doi | https://doi.org/10.1091/mbc.e07-05-0484 | - |
Aparece en las colecciones: | Artículos: Genética y Microbiología |
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madrid_et_al_2007-.pdf | 1,34 MB | Adobe PDF | Visualizar/Abrir |
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