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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Lopes, J.S. | - |
dc.contributor.author | Ivanova, E. | - |
dc.contributor.author | Ruiz, S. | - |
dc.contributor.author | Andrews, S. | - |
dc.contributor.author | Kelsey, G. | - |
dc.contributor.author | Coy, P. | - |
dc.contributor.other | Facultades, Departamentos, Servicios y Escuelas::Departamentos de la UMU::Fisiología | es |
dc.contributor.other | Epigenetics Programme. Babraham Institute. Cambridge, U.K. | es |
dc.contributor.other | Bioinformatics Group. Babraham Institute. Cambridge, U.K. | es |
dc.contributor.other | Centre for Trophoblast Research. Cambridge University. U.K. | es |
dc.date.accessioned | 2024-01-29T08:44:59Z | - |
dc.date.available | 2024-01-29T08:44:59Z | - |
dc.date.issued | 2022-12-18 | - |
dc.identifier.citation | International Journal of Molecular Sciences 2022, 23(24), 16158 | es |
dc.identifier.issn | 1661-6596 | - |
dc.identifier.uri | http://hdl.handle.net/10201/137902 | - |
dc.description | © 2022. The authors. This document is made available under the CC-BY 4.0 license http://creativecommons.org/licenses/by /4.0/ This document is the accepted version of a published work that appeared in final form in International Journal of Molecular Sciences | es |
dc.description.abstract | Controlled ovarian stimulation is a necessary step in some assisted reproductive procedures allowing a higher collection of female gametes. However, consequences of this stimulation for the gamete or the offspring have been shown in several mammals. Most studies used comparisons between oocytes from different donors, which may contribute to different responses. In this work, we use the bovine model in which each animal serves as its own control. DNA methylation profiles were obtained by single-cell whole-genome bisulfite sequencing of oocytes from pre-ovulatory unstimulated follicles compared to oocytes from stimulated follicles. Results show that the global percentage of methylation was similar between groups, but the percentage of methylation was lower for non-stimulated oocytes in the imprinted genes APEG3, MEG3, and MEG9 and higher in TSSC4 when compared to stimulated oocytes. Differences were also found in CGI of imprinted genes: higher methylation was found among non-stimulated oocytes in MEST (PEG1), IGF2R, GNAS (SCG6), KvDMR1 ICR UMD, and IGF2. In another region around IGF2, the methylation percentage was lower for non-stimulated oocytes when compared to stimulated oocytes. Data drawn from this study might help to understand the molecular reasons for the appearance of certain syndromes in assisted reproductive technologies-derived offspring. | es |
dc.format | application/pdf | es |
dc.format.extent | 19 | es |
dc.language | eng | es |
dc.publisher | MDPI | es |
dc.relation | Ambito Nacional. Entidad financiadora: Proyectos de I+D+I, del Programa Estatal de Investigación, Desarrollo e Innovación Orientada a los retos de la sociedad. Nombre: Obtención de animales sanos mediante técnicas de reproducción asistida basadas en condiciones fisiológicas. Ref. AGL2015-66341-R. - Ambito Europeo. Entidad financiadora: European Comission. Research Executive Agency (REA). European Joint Doctorate in Biology and Technology of Reproductive Health. Marie Sklodowska-Curie Innovative Training Network. Ref. REP-BIOTECH 675526. - Ambito Nacional. Entidad financiadora: Agencia de Ciencia y Tecnología de la Región de Murcia. Fundación Séneca Ayudas a los Grupos y Unidades de Excelencia Científica de la Región de Murcia. Nombre: From assisted reproductive technologies onwards: their impact on preimplantation epigenetic reprogramming and their use in the generation of CRISPR-Cas9 transgenic animals. Ref. 20040/GERM/16. | es |
dc.rights | info:eu-repo/semantics/openAccess | es |
dc.rights | Atribución 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject | Oocyte | es |
dc.subject | DNA methylation | es |
dc.subject | Ovarian stimulation | es |
dc.subject | ART | es |
dc.subject | Epigenetics | es |
dc.title | Effect of superovulation treatment on oocyte´s DNA methylation. | es |
dc.type | info:eu-repo/semantics/article | es |
dc.relation.publisherversion | https://www.mdpi.com/1422-0067/23/24/16158 | es |
dc.identifier.doi | https://doi.org/10.3390/ijms232416158 | - |
Aparece en las colecciones: | Artículos: Fisiología |
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Lopes JS et al 2022 Int J Mol Sci.pdf | 5,82 MB | Adobe PDF | Visualizar/Abrir |
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