Por favor, use este identificador para citar o enlazar este ítem:
https://doi.org/10.14670/HH-18-655
Twittear
Título: | Spatial transcriptomic analysis of tumour with high and low CAIX expression in TNBC tissue samples using GeoMx™ RNA assay |
Fecha de publicación: | 2024 |
Editorial: | Universidad de Murcia, Departamento de Biologia Celular e Histiologia |
Cita bibliográfica: | Histology and Histopathology Vol. 39, nº2 (2024) |
ISSN: | 0213-3911 1699-5848 |
Materias relacionadas: | CDU::6 - Ciencias aplicadas::61 - Medicina::616 - Patología. Medicina clínica. Oncología |
Palabras clave: | Breast cancer CAIX Hypoxia gene expression Nanostring nCounter techonology transcriptomics HIF-1α Apoptosis BCL2 Infiltrating macrophages CD68 Lymphocytes CD3 Stromal microenvironment |
Resumen: | Purpose. Prognostic significance and gene signatures associated with carbonic anhydrase IX (CAIX) was investigated in triple negative breast cancer (TNBC) patients. Methods. Immunohistochemistry (IHC) for CAIX was performed in tissue microarrays (TMAs) of 136 TNBC patients. In a subset of 52 patients Digital Spatial Profiler (DSP) was performed in tumour (pancytokeratin+) and stroma (pan-cytokeratin-). Differentially expressed genes (DEGs) with P<0.05 and log2 fold change (FC)>(±0.25 and ±0.3, for tumour and stromal compartment, respectively) were identified. Four genes were validated at the protein level. Result. Cytoplasmic CAIX expression was independently associated with poor recurrence free survival in TNBC patients [hazard ratio (HR)=6.59, 95% confidence interval (CI): 1.47-29.58, P=0.014]. DEG analysis identified 4 up-regulated genes (CD68, HIF1A, pan-melanocyte, and VSIR) in the tumour region and 9 down-regulated genes in the stromal region (CD86, CD3E, MS4A1, BCL2, CCL5, NKG7, PTPRC, CD27, and FAS) when low versus high CAIX expression was explored. Employing IHC, high CD68 and HIF-1α was associated with poorer prognosis and high BCL2 and CD3 was associated with good prognosis. Conclusions. DSP technology identified DEGs in TNBC. Selected genes validated by IHC showed involvement of CD3 and BCL2 expression within stroma and HIF-1α, and CD68 expression within tumour. However, further functional analysis is warranted |
Autor/es principal/es: | Shamis, Suad A.K. Savioli, Francesca Ammar, Aula Al Badran, Sara S.F. Hatthakarnkul, Phimmada Leslie, Holly Mallon, Elizabeth E.A. Jamieson, Nigel B. McMillan, Donald C Edwards, Joanne |
URI: | http://hdl.handle.net/10201/137894 |
DOI: | https://doi.org/10.14670/HH-18-655 |
Tipo de documento: | info:eu-repo/semantics/article |
Número páginas / Extensión: | 24 |
Derechos: | info:eu-repo/semantics/openAccess Attribution-NonCommercial-NoDerivatives 4.0 Internacional |
Aparece en las colecciones: | Vol.39, nº2 (2024) |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
29a1930a-00bd-40f9-8d23-e0aa1a87d24f-5.pdf | 10,33 MB | Adobe PDF | Visualizar/Abrir |
Este ítem está sujeto a una licencia Creative Commons Licencia Creative Commons