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Título: Developing the rate equations for two enzymatic Ping-Pong reactions in series: Application to the bio-synthesis of Bis(2-ethylhexyl) azelate
Fecha de publicación: 15-sep-2020
Editorial: Elsevier
ISSN: 1369-703X
Palabras clave: Kinetic equations
Ping-Pong in series
Dicarboxylic esters
Bis(2-ethylhexyl) azelate
Resumen: In this work, the rate equations of two simultaneous bisubstrate Ping-Pong in series reactions have been developed for the first time. To obtain these equations, the approximation to the stationary state has been applied and, for the total balance of enzyme, all the intermediate complexes of the two reactions, which are present simultaneously in the reaction medium, have been taken into account. To check the kinetic equations obtained, the synthesis of bis(2-ethylhexyl) azelate by transesterification from diethyl azelate and 2-ethylhexanol, in the presence of the immobilized lipase Novozym@ 435, has been used as reaction model. The reaction has been carried out in solvent-free conditions in a batch reactor. A design model of the reactor has also been developed and solved by applying a numerical method. The model equations have been implemented in user’s software in Visual Basic for Applications. In that software a routine, based on a very well-known procedure to minimize the sum of the square errors, has also been implemented. By using this software, the model parameters have been determined by fitting the experimental data to the model. A high determination coefficient was obtained, which validated both the kinetic equations and the reactor’s design model.
Autor/es principal/es: Gómez, M.
Murcia, M.D.
Serrano-Arnaldos, M.
Gómez, E.
Gómez, J.L.
Hidalgo, A.M.
Máximo, M.F.
Facultad/Departamentos/Servicios: Facultades, Departamentos, Servicios y Escuelas::Departamentos de la UMU::Ingeniería Química
Versión del editor: https://www.sciencedirect.com/science/article/pii/S1369703X2030245X?via%3Dihub
URI: http://hdl.handle.net/10201/137791
DOI: https://doi.org/10.1016/j.bej.2020.107691
Tipo de documento: info:eu-repo/semantics/article
Número páginas / Extensión: 33
Derechos: info:eu-repo/semantics/openAccess
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
Descripción: ©2020. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ This document is the Submitted version of a Published Work that appeared in final form in Biochemical Engineering Journal.. To access the final edited and published work see https://doi.org/10.1016/j.bej.2020.107691
Aparece en las colecciones:Artículos: Ingeniería Química

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