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10.1038/cdd.2015.176
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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Martín-Sáncez, Fátima | - |
dc.contributor.author | Diamon, Catherine | - |
dc.contributor.author | Zeitler, Marcel | - |
dc.contributor.author | Gomez-Sanchez, Ana | - |
dc.contributor.author | Baroja-Mazo, Alberto | - |
dc.contributor.author | Bagnall, James | - |
dc.contributor.author | Spiller, David | - |
dc.contributor.author | White, Michael | - |
dc.contributor.author | Daniels, Michael J. D. | - |
dc.contributor.author | Mortellaro, Alessandra | - |
dc.contributor.author | Peñalver, Marcos | - |
dc.contributor.author | Paszek, Pawel | - |
dc.contributor.author | Steringer, Julia P. | - |
dc.contributor.author | Nickel, Walter | - |
dc.contributor.author | Brough, David | - |
dc.contributor.author | Pelegrin Vivancos, Pablo | - |
dc.contributor.other | Facultades, Departamentos, Servicios y Escuelas::Departamentos de la UMU::Bioquímica y Biología Molecular B e Inmunología | es |
dc.date.accessioned | 2024-01-25T09:50:12Z | - |
dc.date.available | 2024-01-25T09:50:12Z | - |
dc.date.issued | 2016 | - |
dc.identifier.citation | Cell Death & Differentiation, volumen 23, nº 7, año 2016, páginas: 1219-1231. | es |
dc.identifier.issn | 1476-5403 | - |
dc.identifier.issn | 1350-9047 | - |
dc.identifier.uri | http://hdl.handle.net/10201/137761 | - |
dc.description | ©2016. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ This document is the Accepted version of a Published Work that appeared in final form in Cell Death & Differentiation,. To access the final edited and published work see https://doi.org/10.1038/cdd.2015.176 | es |
dc.description.abstract | Interleukin (IL)-1β is a critical regulator of the inflammatory response. IL-1β is not secreted through the conventional ER-Golgi route of protein secretion and to-date its mechanism of release has been unknown. Crucially its secretion depends upon the processing of a precursor form following the activation of the multi-molecular inflammasome complex. Using a novel and reversible pharmacological inhibitor of the IL-1β release process, in combination with biochemical, biophysical and real-time single-cell confocal microscopy with macrophage cells expressing Venus labelled IL-1β, we have discovered that the secretion of IL-1β after inflammasome activation requires membrane permeabilisation, and occurs in parallel with the death of the secreting cell. Thus in macrophages the release of IL-1β in response to inflammasome activation appears to be a secretory process independent of non-specific leakage of proteins during cell death. The mechanism of membrane permeabilisation leading to IL-1β release is distinct from the unconventional secretory mechanism employed by its structural homologues FGF2 or IL-1α, a process that involves the formation of membrane pores but does not result in cell death. These discoveries reveal key processes at the initiation of an inflammatory response and deliver new insights into mechanisms of protein release. | es |
dc.format | application/pdf | es |
dc.format.extent | 48 | es |
dc.language | eng | es |
dc.publisher | Nature | es |
dc.relation | Instituto Salud Carlos III (CD12/00523), BBSRC David Phillips Research Fellowship (BB/I017976/1), Instituto Salud Carlos III-FEDER (EMER07/049, PS09/00120, PS13/00174), European Research Council (ERC-2013-CoG 614578), MRC (MR/K015885/1), BBSRC (BB/K003097/1), European Union Seventh Framework Programme (FP7/2012-2017, grant agreement n° 305564), German Research Council (DFG-SFB 638, DFG-SFB/TRR 83, DFG- GRK1188), AID-NET program of the Federal Ministry for Education and Research of Germany, DFG cluster of excellence CellNetworks. | es |
dc.rights | info:eu-repo/semantics/openAccess | es |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Citocinas | es |
dc.subject | Macrófagos | es |
dc.subject | Inflamación | es |
dc.subject | Inflammasoma | es |
dc.subject.other | CDU::6 - Ciencias aplicadas | es |
dc.title | Inflammasome-dependent IL-1β release depends upon membrane permeabilisation | es |
dc.title.alternative | IL-1β release via membrane permeabilisation | es |
dc.type | info:eu-repo/semantics/article | es |
dc.relation.publisherversion | https://www.nature.com/articles/cdd2015176 | es |
dc.identifier.doi | 10.1038/cdd.2015.176 | - |
Aparece en las colecciones: | Artículos: Bioquímica y Biología Molecular "B" e Inmunología |
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2016 CDD.pdf | Versión aceptada del artículo | 5,02 MB | Adobe PDF | Visualizar/Abrir |
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