Por favor, use este identificador para citar o enlazar este ítem:
10.4049/jimmunol.1301676
Twittear
Título: | Apoptosis-associated speck-like protein containing CARD forms specks but does not activate caspase-1 in the absence of NLRP3 during macrophage swelling |
Otros títulos: | ASC speck formation during cell swelling |
Fecha de publicación: | 2015 |
Editorial: | The American Association of Immunologists, Inc. |
Cita bibliográfica: | The Journal of Immunology, volumen 194, nº 3, año 2015, páginas 1261-1273 |
ISSN: | 1550-6606 0022-1767 |
Materias relacionadas: | CDU::6 - Ciencias aplicadas |
Palabras clave: | Macrófagos Citocinas Inflamación Volumen celular Inflamasoma |
Resumen: | Apoptosis-associated speck-like protein containing a CARD (ASC) is a key adaptor molecule required for inflammatory processes. ASC acts by bridging NLRP proteins, such as NLRP3, with pro-caspase-1 within the inflammasome complex that subsequently results in the activation of caspase-1 and the secretion of interleukin (IL)-1b and IL-18. In response to bacterial infection, ASC also forms specks by self-oligomerization to activate caspase-1 and induce pyroptosis. Hitherto the role of these specks in NLRP3 inflammasome activation in response to danger signals is largely unexplored. Here we report that under hypotonic conditions, ASC formed specks independently of NLRP3 that did not activate caspase-1. These specks were not associated with pyroptosis and were controlled by Transient Receptor Potential Vanilloid 2 channel mediated signaling. However, interaction with NLRP3 enhanced ASC speck formation leading to fully functional inflammasomes and caspase-1 activation. This study reveals that the ASC speck could present different oligomerization assemblies and represents an essential step in the activation of functional NLRP3 inflammasomes. |
Autor/es principal/es: | Compan, Vincent Martín-Sánchez, Fátima Baroja-Mazo, Alberto López-Castejón, Gloria Gomez, Ana I. Verkhratsky, Alexei Brough, David Pelegrin Vivancos, Pablo |
Facultad/Departamentos/Servicios: | Facultades, Departamentos, Servicios y Escuelas::Departamentos de la UMU::Bioquímica y Biología Molecular B e Inmunología |
Versión del editor: | https://journals.aai.org/jimmunol/article/194/3/1261/108974/Apoptosis-Associated-Speck-like-Protein-Containing |
URI: | http://hdl.handle.net/10201/137759 |
DOI: | 10.4049/jimmunol.1301676 |
Tipo de documento: | info:eu-repo/semantics/article |
Número páginas / Extensión: | 51 |
Derechos: | info:eu-repo/semantics/openAccess Attribution-NonCommercial-NoDerivatives 4.0 Internacional |
Descripción: | ©2015. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ This document is the Submitted version of a Published Work that appeared in final form in The Journal of Immunology. To access the final edited and published work see https://doi.org/10.4049/jimmunol.1301676 |
Aparece en las colecciones: | Artículos: Bioquímica y Biología Molecular "B" e Inmunología |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
2015 JI ASC.pdf | Versión aceptada del artículo | 1,66 MB | Adobe PDF | Visualizar/Abrir |
Este ítem está sujeto a una licencia Creative Commons Licencia Creative Commons