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10.1074/jbc.M610351200
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Título: | Pannexin-1 couples to maitotoxin and nigericin- induced il-1β release through a dye-uptake independent pathway |
Fecha de publicación: | 2007 |
Editorial: | Elsevier |
Cita bibliográfica: | Journal of Biological Chemistry, volumen 282, nº 4, año 2007, páginas 2386-2394 |
ISSN: | 1083-351X 0021-9258 |
Materias relacionadas: | CDU::6 - Ciencias aplicadas |
Palabras clave: | Panexina Citocinas Macrófagos |
Resumen: | Pannexin-1 is a recently identified membrane protein that can act as a non-selective pore permeable to dyes such as ethidium when ectopically expressed. Blockade of pannexin-1 in macrophage endogenously expressing the ATP-gated P2X7 receptor (P2X7R) blocks the initial dye uptake, but not the ionic current, and also blocks processing and release of interleukin-1β (IL-1β) in response to P2X7R activation. These results suggest that pannexin-1 may be a hemichannel activated by the P2X7R to provide the conduit for dye uptake and downstream signalling to processing and release of IL-1β. We have pursued this hypothesis by measuring dye uptake and IL-1β processing and release in mouse J774 macrophage in response to P2X7R activation and to maitotoxin and nigericin, two agents considered to evoke IL-1β release via the same mechanism. Experiments were carried out over time periods during which no LDH was released from cells in order to examine only non-cytolytic pathways. P2X7R activation evoked dye-uptake that could be separated into two components by pannexin-1 inhibition: an initial rapid phase and a slower pannexin-1- independent phase. Maitotoxin evoked dye uptake was unaltered by pannexin-1 inhibition. Nigericin did not induce dye uptake. Inhibition of pannexin-1 blocked caspase-1 and IL-1β processing and release in response to all three stimuli. Thus, while pannexin-1 is required for IL-1β release in response to maitotoxin, nigericin and ATP, a mechanism distinct from pannexin-1 hemichannel activation must underlie the former two processes. |
Autor/es principal/es: | Pelegrin, Pablo Surprenant, Annmarie |
Facultad/Departamentos/Servicios: | Facultades, Departamentos, Servicios y Escuelas::Departamentos de la UMU::Bioquímica y Biología Molecular B e Inmunología |
Versión del editor: | https://www.jbc.org/article/S0021-9258(20)72108-3/fulltext |
URI: | http://hdl.handle.net/10201/137733 |
DOI: | 10.1074/jbc.M610351200 |
Tipo de documento: | info:eu-repo/semantics/article |
Número páginas / Extensión: | 21 |
Derechos: | info:eu-repo/semantics/openAccess Attribution-NonCommercial-NoDerivatives 4.0 Internacional |
Descripción: | ©2007. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ This document is the Accepte version of a Published Work that appeared in final form in Journal of Biological Chemistry. To access the final edited and published work see https://doi.org/10.1074/jbc.M610351200 |
Aparece en las colecciones: | Artículos: Bioquímica y Biología Molecular "B" e Inmunología |
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2007 JCB.pdf | Versión aceptada del artículo | 1,54 MB | Adobe PDF | Visualizar/Abrir |
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