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10.1006/fsim.2001.0347
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Título: | Phylogeny of cytokines: molecular cloning and expression analysis of sea bass Dicentrarchus labrax interleukin-1b |
Fecha de publicación: | 2001 |
Cita bibliográfica: | Fish & Shellfish Immunology, volumen 11, nº 8, año 2001, páginas 711-726. |
ISSN: | 1050-4648 |
Materias relacionadas: | CDU::6 - Ciencias aplicadas |
Palabras clave: | Interleucina-1 Peces Lubina Citocinas Evolution Leucocitos |
Resumen: | In this paper the cloning of interleukin-1b (IL-1b) from the fish Dicentrarchus labrax (sea bass) is described. Using degenerate primers designed from known IL-1b sequences, a cDNA fragment was amplified by PCR and elongated by 3’ and 5’ RACE to give the full-length coding sequence for sea bass IL-1. The cDNA is 1292 bp, lacks a putative ICE cut site, and codes for a deduced peptide of 29.4 kDa with a pI of 5.1. Sequence analysis showed highest amino acid similarity with rainbow trout (62%), Xenopus (46%), and carp (45.5%) IL-1b sequences. Expression studies show that sea bass IL-1b can be upregulated by bacterial lipopolysaccharide both in vitro and in vivo in leucocytes from blood, head-kidney, spleen, gills and liver, whereas the IL-1b transcript was not detectable in thymus and gut-associated lymphoid tissue. Northern blot analysis with head-kidney leucocyte RNA showed a main LPS- upregulated band at 1.3 kb, and two minor bands at 0.9 and 3.0 kb, respectively. Phylogenetic comparisons with IL-1b from other vertebrates is presented. |
Autor/es principal/es: | Scapigliati, G. Buonocore, F. Bird, S. Zou, J. Pelegrín, Pablo Falasca, C. Prugnoli, D. Secombes, C. J. |
Facultad/Departamentos/Servicios: | Facultades, Departamentos, Servicios y Escuelas::Departamentos de la UMU::Bioquímica y Biología Molecular B e Inmunología |
Versión del editor: | https://www.sciencedirect.com/science/article/pii/S105046480190347X?via%3Dihub |
URI: | http://hdl.handle.net/10201/137724 |
DOI: | 10.1006/fsim.2001.0347 |
Tipo de documento: | info:eu-repo/semantics/article |
Número páginas / Extensión: | 33 |
Derechos: | info:eu-repo/semantics/openAccess Attribution-NonCommercial-NoDerivatives 4.0 Internacional |
Descripción: | ©2001. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ This document is the Accepted version of a Published Work that appeared in final form in Fish & Shellfish Immunology. To access the final edited and published work see https://doi.org/10.1006/fsim.2001.0347 |
Aparece en las colecciones: | Artículos: Bioquímica y Biología Molecular "B" e Inmunología |
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Fichero | Descripción | Tamaño | Formato | |
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2001 Fish & Shellfish Immunology Scapigliati.pdf | Versión aceptada del artículo | 2,83 MB | Adobe PDF | Visualizar/Abrir |
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