Por favor, use este identificador para citar o enlazar este ítem: https://doi.org/10.1016/j.biocel.2007.06.005

Título: Effects of folate cycle disruption by the green tea polyphenol epigallocatechin-3-gallate
Fecha de publicación: 2007
Cita bibliográfica: International Journal of Biochemistry and Cell Biology. Volumen: 39 ,Nº12 , Año: 2007, Pags: 2215-2225
ISSN: 1357-2725
Materias relacionadas: CDU::5 - Ciencias puras y naturales::57 - Biología::577 - Bioquímica. Biología molecular. Biofísica
Palabras clave: Fólico, acido
Resumen: We demonstrate that the tea polyphenol, epigallocatechin-3-gallate, is an efficient inhibitor of human dihydrofolate reductase. Like other antifolate compounds, epigallocatechin-3-gallate acts by disturbing folic acid metabolism in cells, causing the inhibition of DNA and RNA synthesis and altering DNA methylation. Epigallocatechin-3-gallate was seen to inhibit the growth of a human colon carcinoma cell line in a concentration and time dependent manner. Rescue experiments using leucovorin and hypoxanthine-thymine medium were the first indication that epigallocatechin-3-gallate could disturb the folate metabolism within cells. Epigallocatechin-3-gallate increased the uptake of [H-3]-thymidine and showed synergy with 5-fluorouracil, while its inhibitory action was strengthened after treatment with hypoxanthine, which indicates that epigallocatechin-3-gallate decreases the cellular production of nucleotides, thus, disturbing DNA and RNA synthesis. In, addition to its effects on nucleotide biosynthesis, antifolate treatment has been linked to a decrease in cellular methylation. Here, we observed that epigallocatechin-3-gallate altered the p16 methylation pattern from methylated to unmethylated as a result of folic acid deprivation. Finally, we demonstrate that epigallocatechin-3-gallate causes adenosine to be released from the cells because it disrupts the purine metabolism. By binding to its specific receptors, adenosine can modulate different signalling pathways. This proposed mechanism should help us to understand most of the molecular and cellular effects described for this tea polyphenol
Autor/es principal/es: Navarro Perán, Encarnación María
Cabezas Herrera, Juan
Sánchez del Campo Ferrer, Luis
Rodríguez López, José Neptuno
Facultad/Departamentos/Servicios: Facultades, Departamentos, Servicios y Escuelas::Departamentos de la UMU::Bioquímica y Biología Molecular A
Versión del editor: https://doi.org/10.1016/j.biocel.2007.06.005
URI: http://hdl.handle.net/10201/137685
DOI: https://doi.org/10.1016/j.biocel.2007.06.005
Tipo de documento: info:eu-repo/semantics/article
Número páginas / Extensión: 37
Derechos: info:eu-repo/semantics/openAccess
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
Descripción: ©<2007>. This is the Accepted manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ This document is the Accepted version of a Published Work that appeared in final form in The International Journal of Biochemistry & Cell Biology. To access the final edited and published work see https://doi.org/10.1016/j.biocel.2007.06.005
Aparece en las colecciones:Artículos: Bioquímica y Biología Molecular "A"

Ficheros en este ítem:
Fichero Descripción TamañoFormato 
International journal B&CB - 2007 - Navarro-Peran.pdf619,41 kBAdobe PDFVista previa

Este ítem está sujeto a una licencia Creative Commons Licencia Creative Commons Creative Commons