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10.3390/children10081423.


Título: | Colostrum Features of Active and Recovered COVID-19 Patients Revealed Using Next-Generation Proteomics Technique, SWATH-MS |
Fecha de publicación: | 2023 |
Cita bibliográfica: | Children |
ISSN: | 2227-9067 |
Palabras clave: | Breastfeeding Colostrum SARS-CoV-2 COVID-19 Proteomics |
Resumen: | Colostrum performs nutritional, anti-inflammatory and anti-infective functions and promotes immune system formation and organ development. The new coronavirus, SARS-CoV-2, has generated concerns about viral transmission through human milk, with a lack of evidence about human milk’s protective effects against the infection. This study aimed at analyzing presence of the virus and at identifying the protein expression profile of human colostrum in active and COVID-19- recovered patients. Colostrum samples were collected from women with COVID-19 (n = 3), women recently recovered from the infection (n = 4), and non-infected women (n = 5). The samples were analyzed by means of RT-qPCR to determine presence of the virus and using SWATH-MS for proteomic analysis. Proteomic results were then analyzed using bioinformatic methods. The viral tests were negative for SARS-CoV-2 in the colostrum from COVID-19 patients. The proteomic analysis identified 301 common proteins in all samples analyzed. Nineteen proteins were upregulated and 7 were downregulated in the COVID-19 group versus the control samples, whereas 18 were upregulated and 7 were downregulated when comparing the COVID-19 group to the recovered group. Eleven proteins were biomarkers of active COVID-19 infection. Ten were upregulated: ACTN1, CD36, FAM3B, GPRC5B, IGHA2, IGK, PLTP, RAC1, SDCBP and SERPINF1, and one was downregulated: PSAP. These proteins are mainly related to immunity, inflammatory response and protein transport. In conclusion, the results of this study suggest that colostrum is not a vehicle for mother-to-child SARS-CoV-2 transmission. Moreover, the colostrum’s proteome of active and recuperated patients indicate that it could provide immune benefits to infants. |
Autor/es principal/es: | Hernández-Caravaca, Ivan Moros Nicolás, Carla González-Brusi, Leopoldo Romero de Ávila, Mª José De Paco Matallana, Catalina Pelegrín, Pablo Castaño-Molina, Mª Angeles Díaz-Meca, Lucía Sánchez-Romero, Javier Martínez-Alarcón, Laura Avilés, Manuel Izquierdo-Rico, Mª José |
Facultad/Departamentos/Servicios: | Facultades, Departamentos, Servicios y Escuelas::Departamentos de la UMU::Biología Celular e Histología |
URI: | http://hdl.handle.net/10201/137631 |
DOI: | 10.3390/children10081423. |
Tipo de documento: | info:eu-repo/semantics/article |
Número páginas / Extensión: | 19 |
Derechos: | info:eu-repo/semantics/openAccess Atribución 4.0 Internacional |
Descripción: | ©2023. This manuscript version is made available under the CC-BY 4.0 license http://creativecommons.org/licenses/by /4.0/ This document is the Published, version of a Published Work that appeared in final form inChildren. To access the final edited and published work see https://doi.org/10.3390/children10081423 |
Aparece en las colecciones: | Artículos: Biología Celular e Histología |
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Fichero | Descripción | Tamaño | Formato | |
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Hernández-Caravaca_2023_Children.pdf | 3,3 MB | Adobe PDF | ![]() Visualizar/Abrir |
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