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10.1007/s10863-005-6635-z
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Título: | Intracellular Ca2+ Pools and Fluxes in Cardiac Muscle-Derived H9c2 Cells |
Fecha de publicación: | ago-2005 |
Cita bibliográfica: | Journal of Bioenergetics and Biomembranes 2005 Aug;37(4):249-59.doi: 10.1007/s10863-005-6635-z. |
ISSN: | 1573-6881 0145-479X |
Materias relacionadas: | CDU::6 - Ciencias aplicadas |
Palabras clave: | Ca2+ pools Thapsigargin Vasopressin Sarco-endoplasmic reticulum Cytoplasmic Ca2+ Mitochondrial Ca2+ Cardiomyocyte |
Resumen: | Relevant Ca(2+) pools and fluxes in H9c2 cells have been studied using fluorescent indicators and Ca(2+)-mobilizing agents. Vasopressin produced a cytoplasmic Ca(2+) peak with half-maximal effective concentration of 6 nM, whereas thapsigargin-induced Ca(2+) increase showed half-maximal effect at 3 nM. Depolarization of the mitochondrial inner membrane by protonophore was also associated with an increase in cytoplasmic Ca(2+). Ionomycin induced a small and sustained depolarization, while thapsigargin had a small but transient effect. The thapsigargin-sensitive Ca(2+) pool was also sensitive to ionomycin, whereas the protonophore-sensitive Ca(2+) pool was not. The vasopressin-induced cytoplasmic Ca(2+) signal, which caused a reversible discharge of the sarco-endoplasmic reticulum Ca(2+) pool, was sensed as a mitochondrial Ca(2+) peak but was unaffected by the permeability transition pore inhibitor cyclosporin A. The mitochondrial Ca(2+) peak was affected by cyclosporin A when the Ca(2+) signal was induced by irreversible discharge of the intracellular Ca(2+) pool, i.e., adding thapsigargin. These observations indicate that the mitochondria interpret the cytoplasmic Ca(2+) signals generated in the reticular store. |
Autor/es principal/es: | Lax Pérez, Antonio Manuel Soler, Fernando Fernandez Belda, Francisco |
URI: | http://hdl.handle.net/10201/137584 |
DOI: | 10.1007/s10863-005-6635-z |
Tipo de documento: | info:eu-repo/semantics/article |
Número páginas / Extensión: | 11 |
Derechos: | info:eu-repo/semantics/openAccess Attribution-NonCommercial-NoDerivatives 4.0 Internacional |
Descripción: | ©2005. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ This document is the Accepted version of a Published Work that appeared in final form in Journal of Bioenergetics and Biomembranes. To access the final edited and published work see https://doi.org/ 10.1007/s10863-005-6635-z |
Aparece en las colecciones: | Artículos: Medicina |
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