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Título: Daily rhythms of toxicity and effectiveness of anaesthetics (MS222 and Eugenol) in zebrafish (Danio rerio)
Fecha de publicación: 2011
Cita bibliográfica: Chronobiology International
Palabras clave: Chronotoxicity
Anaesthetics
Eugenol
MS-222
Zebrafish
Daily rhythms
Swimming activity
Resumen: Although the chronotoxicity of xenobiotics is relatively well known in mammals, the existence of daily rhythms of drug toxicity and effectiveness in fish have been neglected to date. The aim of this research was to investigate the influence of the time (middle of the light phase, ML vs middle of the dark phase, MD) of exposure to two anesthetics (MS-222 or clove oil) commonly used in fish on the median lethal concentration (LC50) and swimming activity of zebrafish (Danio rerio). To this end, adult zebrafish were kept under a 12:12h LD cycle and exposed to different concentrations of anesthetic for 15 minutes at ML or MD. The LC 50 calculations were performed using the Spearman Karber program, while swimming activity was video recorded and analyzed with specialized software. In the trials zebrafish exhibited a mostly diurnal activity pattern (77.9% of activity occurring during daytime). The acute toxicity and mortality caused by MS-222 and eugenol varied with the time of exposure. In the case of MS-222, the LC50 was 170. 6 ± 7.4 mg/L in fish exposed at ML, and 215.6 ± 3.9 mg/L at MD while the LC50 of eugenol was 70.3 ± 3.1 mg/L at ML, and 104.9 ± 5.4 mg/L at MD. Exposure to sublethal concentrations of MS-222 and eugenol also altered the swimming patterns of zebrafish in a different manner depending on the time of exposure. Thus, the time required for decreasing swimming activity during exposure to anaesthetics was shorter at ML than at MD whereas the recovery period was longer during the day. In conclusion, these results revealed that the toxicity and effectiveness of both anesthetics is highest during daytime, coinciding with the active phase of fish and suggesting a link between the daily rhythms of behaviour and toxicity.
Autor/es principal/es: Sánchez Vázquez, Francisco J.
Terry, Marta I.
Felizardo, Viviane O.
Vera, L. M.
Facultad/Departamentos/Servicios: Facultades, Departamentos, Servicios y Escuelas::Departamentos de la UMU::Fisiología
URI: http://hdl.handle.net/10201/137229
DOI: https://doi.org/10.3109/07420528.2010.538105
Tipo de documento: info:eu-repo/semantics/article
Número páginas / Extensión: 30
Derechos: info:eu-repo/semantics/openAccess
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
Descripción: ©2011. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ This document is the Accepted version of a Published Work that appeared in final form in Chronobiology International. To access the final edited and published work see https://doi.org/10.3109/07420528.2010.538105
Aparece en las colecciones:Artículos: Fisiología

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