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Título: Bottlenose dolphins (Tursiops truncatus) do also cast neutrophil extracellular traps against the apicomplexan parasite Neospora caninum
Fecha de publicación: dic-2017
Editorial: Elsevier
Cita bibliográfica: International Journal for Parasitology: Parasites and Wildlife Volume 6, Issue 3, December 2017, Pages 287-294
ISSN: 2213-2244
Palabras clave: Tursiops truncatus
Neutrophil extracellular traps
Innate immunity
Neospora caninum
Resumen: Neutrophil extracellular traps (NETs) are web-like structures composed of nuclear DNA decorated with histones and cytoplasmic peptides which antiparasitic properties have not previously been investigated in cetaceans. Polymorphonuclear neutrophils (PMN) were isolated from healthy bottlenose dolphins (Tursiops truncatus), and stimulated with Neospora caninum tachyzoites and the NETs-agonist zymosan. In vitro interactions of PMN with the tachyzoites resulted in rapid extrusion of NETs. For the demonstration and quantification of cetacean NETs, extracellular DNA was stained by using either Sytox Orange® or Pico Green®. Scanning electron microscopy (SEM) and fluorescence analyses demonstrated PMN-derived release of NETs upon exposure to tachyzoites of N. caninum. Co-localization studies of N. caninum induced cetacean NETs proved the presence of DNA adorned with histones (H1, H2A/H2B, H3, H4), neutrophil elastase (NE), myeloperoxidase (MPO) and pentraxin (PTX) confirming the molecular properties of mammalian NETosis. Dolphin-derived N. caninum-NETosis were efficiently suppressed by DNase I and diphenyleneiodonium (DPI) treatments. Our results indicate that cetacean-derived NETs represent an ancient, conserved and relevant defense effector mechanism of the host innate immune system against N. caninum and probably other related neozoan parasites circulating in the marine environment.
Autor/es principal/es: Villagra-Blanco, R.
Silva, L.M.R.
Aguilella-Segura, A.
Arcenillas Hernández, Irene
Martínez Carrasco-Pleite, Carlos
Seipp, A.
Gärtner, U.
Ruiz de Ybáñez Carnero, María del Rocío
Taubert, A.
Hermosilla, C.
Facultad/Departamentos/Servicios: Facultades, Departamentos, Servicios y Escuelas::Departamentos de la UMU::Sanidad Animal
Versión del editor: https://www.sciencedirect.com/science/article/pii/S2213224417300639?via%3Dihub
URI: http://hdl.handle.net/10201/137040
DOI: https://doi.org/10.1016/j.ijppaw.2017.09.002
Tipo de documento: info:eu-repo/semantics/article
Número páginas / Extensión: 8
Derechos: info:eu-repo/semantics/openAccess
Atribución 4.0 Internacional
Descripción: © 2017 The Authors. Published by Elsevier Ltd on behalf of Australian Society for Parasitology. This is an open access article under the CC BY-NC-ND license (http:// creativecommons.org/licenses/by-nc-nd/4.0/). This document is the published version of a published Work that appeared in final form in International Journal for Parasitology: Parasites and Wildlife
Aparece en las colecciones:Artículos: Sanidad Animal

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