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https://doi.org/10.1128/AEM.01661-21.
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Título: | Genome Mosaicism in Field Strains of Mycoplasma bovis as Footprints of In-Host Horizontal Chromosomal Transfer |
Fecha de publicación: | 11-ene-2022 |
Fecha de defensa / creación: | 18-ago-2021 |
Editorial: | AMERICAN SOCIETY FOR MICROBIOLOGY |
Cita bibliográfica: | Applied and Environmental Microbiology, 88 Issue 1 e01661-21 |
ISSN: | 0099-2240 1098-5336 |
Palabras clave: | Mycoplasma bovis Horizontal gene transfer Mosaic genomes Mobile genetic elements Mycoplasma chromosomal transfer |
Resumen: | Horizontal gene transfer was long thought to be marginal in Mollicutes, but the capacity of some of these wall-less bacteria to exchange large chromosomal regions has been recently documented. Mycoplasma chromosomal transfer (MCT) is an unconventional mechanism that relies on the presence of a functional integrative conjugative element (ICE) in at least one partner and involves the horizontal acquisition of small and large chromosomal fragments from any part of the donor genome, which results in progenies composed of an infinite variety of mosaic genomes. The present study focuses on Mycoplasma bovis, an important pathogen of cattle responsible for major economic losses worldwide. By combining phylogenetic tree reconstructions and detailed comparative genome analyses of 36 isolates collected in Spain (2016 to 2018), we confirmed the mosaic nature of 16 field isolates and mapped chromosomal transfers exchanged between their hypothetical ancestors. This study provides evidence that MCT can take place in the field, most likely during coinfections by multiple strains. Because mobile genetic elements (MGEs) are classical contributors of genome plasticity, the presence of phages, insertion sequences (ISs), and ICEs was also investigated. Data revealed that these elements are widespread within the M. bovis species and evidenced classical horizontal transfer of phages and ICEs in addition to MCT. These events contribute to wide-genome diversity and reorganization within this species and may have a tremendous impact on diagnostic and disease control. |
Autor/es principal/es: | García Galán Pérez, Ana Baranowski, Eric Hygonenq, Marie-Claude Walch, Matilda Croville, Guillaume Citti, Christine Fé Rodríguez, Christian de la Nouvel, Laurent Xavier |
Facultad/Departamentos/Servicios: | Facultades, Departamentos, Servicios y Escuelas::Departamentos de la UMU::Sanidad Animal |
Forma parte de: | Project AGL2016-76568-R |
URI: | http://hdl.handle.net/10201/136883 |
DOI: | https://doi.org/10.1128/AEM.01661-21. |
Tipo de documento: | info:eu-repo/semantics/article |
Número páginas / Extensión: | 15 |
Derechos: | info:eu-repo/semantics/openAccess |
Descripción: | Rights Retained by the Author ASM grants the Author and the employer for whom the Work was created (if applicable) a royalty-free, non-exclusive limited license to: a) post the Work (after publication by ASM) on the Author's personal or university-hosted website. |
Aparece en las colecciones: | Artículos: Sanidad Animal |
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