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https://doi.org/10.1016/j.cell.2017.08.009
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Título: | In Situ Architecture and Cellular Interactions of PolyQ Inclusions |
Fecha de publicación: | 21-sep-2017 |
Editorial: | Elsevier |
Cita bibliográfica: | Cell. Vol. 171, Issue 1, 21 September 2017, Pages 179-187.e10 |
ISSN: | Print: 0092-8674 Electronic: 1097-4172 |
Resumen: | Expression of many disease-related aggregation-prone proteins results in cytotoxicity and the formation of large intracellular inclusion bodies. To gain insight into the role of inclusions in pathology and the in situ structure of protein aggregates inside cells, we employ advanced cryo-electron tomography methods to analyze the structure of inclusions formed by polyglutamine (polyQ)-expanded huntingtin exon 1 within their intact cellular context. In primary mouse neurons and immortalized human cells, polyQ inclusions consist of amyloid-like fibrils that interact with cellular endomembranes, particularly of the endoplasmic reticulum (ER). Interactions with these fibrils lead to membrane deformation, the local impairment of ER organization, and profound alterations in ER membrane dynamics at the inclusion periphery. These results suggest that aberrant interactions between fibrils and endomembranes contribute to the deleterious cellular effects of protein aggregation. |
Autor/es principal/es: | Bäuerlein, Felix Saha, Itika Mishra, Archana Kalemanov, Maria Martinez-Sanchez, Antonio Klein, Rudiger Dudanova, Irina Hipp, Mark Hartl, Ulrich Baumeister, Wolfgang Fernandez-Busnadiego, Ruben |
Facultad/Departamentos/Servicios: | Facultades, Departamentos, Servicios y Escuelas::Departamentos de la UMU::Ingeniería de la Información y las Comunicaciones |
URI: | http://hdl.handle.net/10201/136738 |
DOI: | https://doi.org/10.1016/j.cell.2017.08.009 |
Tipo de documento: | info:eu-repo/semantics/article |
Número páginas / Extensión: | 20 |
Derechos: | info:eu-repo/semantics/openAccess Attribution-NonCommercial-NoDerivatives 4.0 Internacional |
Descripción: | © 2017 Elsevier Inc. This document is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ This document is the accepted version of a published Work that appeared in final form in cell. To access the final edited and published work see https://doi.org/10.1016/j.cell.2017.08.009 |
Aparece en las colecciones: | Artículos: Ingeniería de la Información y las Comunicaciones |
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