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https://doi.org/10.1111/mmi.13874
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Título: | An acetylatable lysine controls CRP function in E. coli. |
Fecha de publicación: | 31-oct-2017 |
Editorial: | John Wiley & Sons |
Cita bibliográfica: | Molecular Microbiology 2018 Jan;107(1):116-131 |
ISSN: | 0950-382X |
Resumen: | Transcriptional regulation is the key to ensuring that proteins are expressed at the proper time and the proper amount. In Escherichia coli, the transcription factor cAMP receptor protein (CRP) is responsible for much of this regulation. Questions remain, however, regarding the regulation of CRP activity itself. Here, we demonstrate that a lysine (K100) on the surface of CRP has a dual function: to promote CRP activity at Class II promoters, and to ensure proper CRP steady state levels. Both functions require the lysine’s positive charge; intriguingly, the positive charge of K100 can be neutralized by acetylation using the central metabolite acetyl phosphate as the acetyl donor. We propose that CRP K100 acetylation could be a mechanism by which the cell downwardly tunes CRPdependent Class II promoter activity, whilst elevating CRP steady state levels, thus indirectly increasing Class I promoter activity. This mechanism would operate under conditions that favor acetate fermentation, such as during growth on glucose as the sole carbon source or when carbon flux exceeds the capacity of the central metabolic pathways. |
Autor/es principal/es: | Davis, Robert Ecija Conesa, Ana Gallego Jara, Julia De Diego Puente, María Teresa Filippova, Ekaterina V. Schilling, Birgit Anderson, Wayne F. Gibson, Bradford W. Cánovas Diaz, Manuel Wolfe, Alan J. Kuffel, Gina |
Facultad/Departamentos/Servicios: | Facultades, Departamentos, Servicios y Escuelas::Departamentos de la UMU::Bioquímica y Biología Molecular B e Inmunología |
Versión del editor: | https://onlinelibrary.wiley.com/doi/10.1111/mmi.13874 |
URI: | http://hdl.handle.net/10201/136581 |
DOI: | https://doi.org/10.1111/mmi.13874 |
Tipo de documento: | info:eu-repo/semantics/article |
Número páginas / Extensión: | 16 |
Derechos: | info:eu-repo/semantics/openAccess Attribution-NonCommercial-NoDerivatives 4.0 International |
Descripción: | ©2017. The authors. This document is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ This document is the accepted version of a Published Work that appeared in final form in Molecular Microbiology. To access the final edited and published work see https://doi.org/10.1111/mmi.13874 |
Aparece en las colecciones: | Artículos: Bioquímica y Biología Molecular "B" e Inmunología |
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