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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Wang, Zhanyu | - |
dc.contributor.author | Zhang, Xiaoping | - |
dc.contributor.author | Liu, Yongying | - |
dc.contributor.author | Shi, Xiaoyan | - |
dc.contributor.author | Li, Lijun | - |
dc.contributor.author | Jia, Yun | - |
dc.contributor.author | Wu, Fangfang | - |
dc.contributor.author | Cui, Haosen | - |
dc.contributor.author | Li, Liang | - |
dc.date.accessioned | 2023-12-01T09:17:02Z | - |
dc.date.available | 2023-12-01T09:17:02Z | - |
dc.date.issued | 2023 | - |
dc.identifier.citation | Histology and Histopathology Vol. 38, nº12 (2023) | es |
dc.identifier.issn | 0213-3911 | - |
dc.identifier.issn | 1699-5848 | - |
dc.identifier.uri | http://hdl.handle.net/10201/136272 | - |
dc.description.abstract | Background. Recent studies have reported the tumor suppressive role of miR-5195-3p in the progression of several cancers, but the potential roles of miR-5195-3p in ovarian cancer (OC) remain largely unknown. Methods. We first analyzed the expression levels of miR-5195-3p in 83 pairs of human OC tissues and adjacent specimens by reverse transcription-quantitative PCR. The correlation of miR-5195-3p/rhomboid domain containing 1 (RHBDD1) and clinicopathological parameters was analyzed by chi-square test. The prognostic value of miR-5195-3p was evaluated by Kaplan-Meier method Cox proportional hazards models. The effects of miR-5195-3p on cell proliferation, cell cycle distribution, migration and invasion were examined by CCK-8 assay, colony formation assay, flow cytometry and transwell assay. Tumor forming was evaluated by nude mice model in vivo. The association between miR-5195-3p and RHBDD1 was verified by luciferase reporter assay. Results. We observed that miR-5195-3p level was remarkably reduced in OC tissues as compared to adjacent tissues. The expression of miR-5195-3p was associated with FIGO stage, depth of invasion and poor survival prognosis in OC patients. Overexpression of miR-5195-3p significantly suppressed cell proliferation, cell cycle G1/S transition, migration and invasion in OC cell lines (SKOV-3 and OVCAR3), while knockdown of miR-5195-3p obtained the opposite results. We further confirmed miR-5195-3p as a negative posttranscriptional modulator of RHBDD1. RHBDD1 expression was upregulated in OC tissues compared with adjacent tissues, which was inversely correlated with miR-5195-3p expression. The expression of RHBDD1 was associated with FIGO stage and distant metastasis. RHBDD1 overexpression reversed the suppressive role of miR-5195-3p on OC cell proliferation, migration and invasion. Consistent with the in vitro results, miR-51953p overexpression decreased the growth of subcutaneously inoculated tumors in nude mice. Conclusions. Taken together, the present results indicated that miR-5195-3p acts a tumor suppressor by targeting RHBDD1 in OC. | es |
dc.format | application/pdf | es |
dc.format.extent | 11 | es |
dc.language | eng | es |
dc.publisher | Universidad de Murcia, Departamento de Biologia Celular e Histiologia | es |
dc.relation | Sin financiación externa a la Universidad | es |
dc.rights | info:eu-repo/semantics/openAccess | es |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Ovarian cancer | es |
dc.subject | miR-5195-3p | es |
dc.subject | RHBDD1 | es |
dc.subject | Prognosis | es |
dc.subject | Cell functions | es |
dc.subject.other | CDU::6 - Ciencias aplicadas::61 - Medicina::616 - Patología. Medicina clínica. Oncología | es |
dc.title | MiR-5195-3p functions as a tumor suppressor by targeting RHBDD1 in ovarian cancer | es |
dc.type | info:eu-repo/semantics/article | es |
dc.identifier.doi | https://doi.org/10.14670/HH-18-595 | - |
Aparece en las colecciones: | Vol.38,nº12 (2023) |
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