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Título: The addition of Lactobacillus spp. negatively affects Mycoplasma bovis viability in bovine cervical mucus
Fecha de publicación: 20-jul-2020
Editorial: BMC
Cita bibliográfica: BMC Veterinary Research 16, 251 (2020)
ISSN: 1746-6148
Palabras clave: In vitro
Mycoplasma bovis
Lactobacillus spp.
Cervical mucus
Resumen: Background Mycoplasma bovis is an important pathogen for the cattle industry worldwide causing significant economic losses. Several transmission routes, including those related to reproduction, have been described. Indeed, the pathogen can colonize the female reproductive tract after artificial insemination (AI) with contaminated semen. Lactobacillus spp.-based probiotics have been used for vaginal dysbiosis treatment in women and cows although their role in controlling cervico-vaginal infections due to M. bovis is unknown. The objective of the present work is to assess the viability of M. bovis (PG45, NCTC 10131) in experimentally contaminated cervical mucus after the addition of Lactobacillus spp. at different concentrations as a competing agent and pH acidifier. Results The addition of probiotic at a concentration higher than 108 colony forming units (CFU/mL had a detrimental effect (P < 0.05) on mycoplasma viability in cervical mucus. This coincided with a significant LAB growth and an important decrease in pH from 8.4 to 5.6 (P < 0.05). However, after the addition of less concentrated probiotic, M. bovis survival was not affected and there was no significant LAB growth despite the drop of pH from 8.4 to 6.73 (P < 0.05). Conclusion The addition of concentrations higher than 108 CFU/mL of Lactobacillus spp. negatively affects M. bovis viability in bovine cervical mucus under in vitro conditions. Although the effect observed on the pathogen viability seems to be related to the pH decrease after LAB proliferation in cervical mucus, further studies are necessary to elucidate if other factors are implicated. Nevertheless, the administration of Lactobacillus spp.-based probiotics might be used in the future to control M. bovis proliferation in the cervico-vaginal tract of cows.
Autor/es principal/es: García-Galán Pérez, Ana
de la Fe Rodríguez, Christian
Gomis Almendro, Jesús
Bataller, Esther
Sánchez López, Antonio
Quereda Torres, Juan José
García Roselló, Empar
Gómez Martín, Ángel
Facultad/Departamentos/Servicios: Facultades, Departamentos, Servicios y Escuelas::Facultades de la UMU::Facultad de Veterinaria
Facultades, Departamentos, Servicios y Escuelas::Departamentos de la UMU::Sanidad Animal
Versión del editor: https://bmcvetres.biomedcentral.com/articles/10.1186/s12917-020-02454-9#citeas
URI: http://hdl.handle.net/10201/136027
DOI: https://doi.org/10.1186/s12917-020-02454-9
Tipo de documento: info:eu-repo/semantics/article
Número páginas / Extensión: 7
Derechos: info:eu-repo/semantics/openAccess
Atribución 4.0 Internacional
Descripción: © The Author(s). 2020 Open Access This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). This document is the Published version of a Published Work that appeared in final form in BMC Veterinary Research. To access the final edited and published work see https://doi.org/10.1186/s12917-020-02454-9
Aparece en las colecciones:Artículos: Sanidad Animal

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