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Título: | Mycoplasma bovis in Spanish Cattle Herds: Two Groups of Multiresistant Isolates Predominate, with One Remaining Susceptible to Fluoroquinolones |
Fecha de publicación: | 7-jul-2020 |
Editorial: | MDPI |
Cita bibliográfica: | Pathogens 2020, 9(7), 545 |
ISSN: | 2076-0817 |
Palabras clave: | Mycoplasma bovis Minimum inhibitory concentration Antimicrobial resistance Mutations Spain |
Resumen: | Mycoplasma bovis is an important bovine pathogen causing pneumonia, mastitis, and arthritis and is responsible for major economic losses worldwide. In the absence of an efficient vaccine, control of M. bovis infections mainly relies on antimicrobial treatments, but resistance is reported in an increasing number of countries. To address the situation in Spain, M. bovis was searched in 436 samples collected from beef and dairy cattle (2016–2019) and 28% were positive. Single-locus typing using polC sequences further revealed that two subtypes ST2 and ST3, circulate in Spain both in beef and dairy cattle, regardless of the regions or the clinical signs. Monitoring of ST2 and ST3 isolates minimum inhibitory concentration (MIC) to a panel of antimicrobials revealed one major difference when using fluoroquinolones (FQL): ST2 is more susceptible than ST3. Accordingly, whole-genome sequencing (WGS) further identified mutations in the gyrA and parC regions, encoding quinolone resistance-determining regions (QRDR) only in ST3 isolates. This situation shows the capacity of ST3 to accumulate mutations in QRDR and might reflect the selective pressure imposed by the extensive use of these antimicrobials. MIC values and detection of mutations by WGS also showed that most Spanish isolates are resistant to macrolides, lincosamides, and tetracyclines. Valnemulin was the only one effective, at least in vitro, against both STs. |
Autor/es principal/es: | García-Galán Pérez, Ana Nouvel, Laurent-Xavier Baranowski, Eric Gómez-Martín, Ángel Sánchez López, Antonio Citti, Christine de la Fe Rodríguez, Christian |
Facultad/Departamentos/Servicios: | Facultades, Departamentos, Servicios y Escuelas::Facultades de la UMU::Facultad de Veterinaria Facultades, Departamentos, Servicios y Escuelas::Departamentos de la UMU::Sanidad Animal |
Versión del editor: | https://www.mdpi.com/2076-0817/9/7/545 |
URI: | http://hdl.handle.net/10201/136026 |
DOI: | https://doi.org/10.3390/pathogens9070545 |
Tipo de documento: | info:eu-repo/semantics/article |
Número páginas / Extensión: | 19 |
Derechos: | info:eu-repo/semantics/openAccess Atribución 4.0 Internacional |
Descripción: | © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).This document is the Accepted version of a Published Work that appeared in final form in Pathogens. To access the final edited and published work see https://doi.org/10.3390/pathogens9070545 |
Aparece en las colecciones: | Artículos: Sanidad Animal |
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García Galán 2020 Pathogens M bovis in Spain.pdf | Artículo | 3,11 MB | Adobe PDF | Visualizar/Abrir |
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