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Título: Mycoplasma bovis in Spanish Cattle Herds: Two Groups of Multiresistant Isolates Predominate, with One Remaining Susceptible to Fluoroquinolones
Fecha de publicación: 7-jul-2020
Editorial: MDPI
Cita bibliográfica: Pathogens 2020, 9(7), 545
ISSN: 2076-0817
Palabras clave: Mycoplasma bovis
Minimum inhibitory concentration
Antimicrobial resistance
Resumen: Mycoplasma bovis is an important bovine pathogen causing pneumonia, mastitis, and arthritis and is responsible for major economic losses worldwide. In the absence of an efficient vaccine, control of M. bovis infections mainly relies on antimicrobial treatments, but resistance is reported in an increasing number of countries. To address the situation in Spain, M. bovis was searched in 436 samples collected from beef and dairy cattle (2016–2019) and 28% were positive. Single-locus typing using polC sequences further revealed that two subtypes ST2 and ST3, circulate in Spain both in beef and dairy cattle, regardless of the regions or the clinical signs. Monitoring of ST2 and ST3 isolates minimum inhibitory concentration (MIC) to a panel of antimicrobials revealed one major difference when using fluoroquinolones (FQL): ST2 is more susceptible than ST3. Accordingly, whole-genome sequencing (WGS) further identified mutations in the gyrA and parC regions, encoding quinolone resistance-determining regions (QRDR) only in ST3 isolates. This situation shows the capacity of ST3 to accumulate mutations in QRDR and might reflect the selective pressure imposed by the extensive use of these antimicrobials. MIC values and detection of mutations by WGS also showed that most Spanish isolates are resistant to macrolides, lincosamides, and tetracyclines. Valnemulin was the only one effective, at least in vitro, against both STs.
Autor/es principal/es: García-Galán Pérez, Ana
Nouvel, Laurent-Xavier
Baranowski, Eric
Gómez-Martín, Ángel
Sánchez López, Antonio
Citti, Christine
de la Fe Rodríguez, Christian
Facultad/Departamentos/Servicios: Facultades, Departamentos, Servicios y Escuelas::Facultades de la UMU::Facultad de Veterinaria
Facultades, Departamentos, Servicios y Escuelas::Departamentos de la UMU::Sanidad Animal
Versión del editor: https://www.mdpi.com/2076-0817/9/7/545
URI: http://hdl.handle.net/10201/136026
DOI: https://doi.org/10.3390/pathogens9070545
Tipo de documento: info:eu-repo/semantics/article
Número páginas / Extensión: 19
Derechos: info:eu-repo/semantics/openAccess
Atribución 4.0 Internacional
Descripción: © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).This document is the Accepted version of a Published Work that appeared in final form in Pathogens. To access the final edited and published work see https://doi.org/10.3390/pathogens9070545
Aparece en las colecciones:Artículos: Sanidad Animal

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