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https://doi.org/10.3390/ani10050837
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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | García-Galán Pérez, Ana | - |
dc.contributor.author | Gómez-Martín, Ángel | - |
dc.contributor.author | Bataller, Esther | - |
dc.contributor.author | Gomis Almendro, Jesús | - |
dc.contributor.author | Sánchez López, Antonio | - |
dc.contributor.author | Gadea Mateos, Joaquín | - |
dc.contributor.author | Vieira, Luis Alberto | - |
dc.contributor.author | García Roselló, Empar | - |
dc.contributor.author | de la Fe Rodríguez, Christian | - |
dc.contributor.other | Facultades, Departamentos, Servicios y Escuelas::Facultades de la UMU::Facultad de Veterinaria | es |
dc.contributor.other | Facultades, Departamentos, Servicios y Escuelas::Departamentos de la UMU::Sanidad Animal | es |
dc.date.accessioned | 2023-11-24T12:00:06Z | - |
dc.date.available | 2023-11-24T12:00:06Z | - |
dc.date.issued | 2020-05-13 | - |
dc.identifier.citation | Animals 2020, 10(5), 837 | es |
dc.identifier.issn | 2076-2615 | - |
dc.identifier.uri | http://hdl.handle.net/10201/136006 | - |
dc.description | © The Authors. 2020. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). This document is the Published version of a Published Work that appeared in final form in Animals. To access the final edited and published work see https://doi.org/10.3390/ani10050837 | es |
dc.description.abstract | Mycoplasma bovis is an important etiologic agent of bovine mycoplasmosis in cattle. Different transmission routes have been described, including those related to reproduction. The presence of mycoplasma in semen has led to its appearance in infection-free areas through artificial insemination (AI). Semen was recently reported to be the initial source of two M. bovis mastitis outbreaks in two closed dairy herds in Finland. This questions the effectiveness of the antimicrobials currently used in semen extenders to control the pathogens in contaminated semen. They should be re-evaluated, or alternative measures to antimicrobials should be tested to obtain M. bovis-free semen. This in vitro study aimed to assess different strategies to reduce the risk of transmission of M. bovis through AI technologies. The viability of M. bovis (PG45, NCTC 10131) in bull semen diluted (DS) in a Tris-citrate-fructose solution was tested, after the addition of enrofloxacin, doxycycline or a Lactobacillus spp.-based probiotic. The data show the susceptibility of the pathogen to the addition of 0.125 μg/mL of enrofloxacin or 0.0625 μg/mL of doxycycline and to the addition of the probiotic at a concentration of 3.24 × 106 colony forming units (CFU)/mL or 3.24 × 108 CFU/mL in DS. The Tris-citrate-fructose medium negatively affected the viability of M. bovis, although this effect was lower than that observed after the addition of the probiotic and antimicrobials (p < 0.05). Our results may support new strategies for reducing the risk of M. bovis transmission through AI. | es |
dc.format | application/pdf | es |
dc.format.extent | 10 | es |
dc.language | eng | es |
dc.publisher | MDPI | es |
dc.relation | This work was supported by the Spanish Ministry of Economy and Competitiveness (Spanish Government) and co-financed by FEDER funds, project AGL2016-76568-R. | es |
dc.rights | info:eu-repo/semantics/openAccess | es |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | In vitro | es |
dc.subject | Viability | es |
dc.subject | Mycoplasma bovis | es |
dc.subject | Diluted bull semen | es |
dc.subject | Enrofloxacin | es |
dc.subject | Doxycycline | es |
dc.subject | Lactobacillus spp. | es |
dc.title | The Addition of Lactobacillus spp., Enrofloxacin or Doxycycline Negatively Affects the Viability of Mycoplasma bovis in Diluted Bovine Semen | es |
dc.type | info:eu-repo/semantics/article | es |
dc.relation.publisherversion | https://www.mdpi.com/2076-2615/10/5/837 | es |
dc.identifier.doi | https://doi.org/10.3390/ani10050837 | - |
Aparece en las colecciones: | Artículos: Sanidad Animal |
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García Galán 2020 Lactobacillus on M bovis semen.pdf | Artículo | 343,69 kB | Adobe PDF | ![]() Visualizar/Abrir |
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