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Campo DC | Valor | Lengua/Idioma |
---|---|---|
dc.contributor.author | Qian, Dongchen | - |
dc.contributor.author | Chen, Yueyue | - |
dc.contributor.author | Qiu, Xusheng | - |
dc.contributor.author | Zhu, Baohua | - |
dc.contributor.author | Zhang, Lin | - |
dc.contributor.author | Yan, Yifeng | - |
dc.contributor.author | Chen, Yixin | - |
dc.date.accessioned | 2023-09-29T09:12:35Z | - |
dc.date.available | 2023-09-29T09:12:35Z | - |
dc.date.issued | 2023 | - |
dc.identifier.citation | Histology and Histopathology Vol. 38, nº10 (2023) | es |
dc.identifier.issn | 0213-3911 | - |
dc.identifier.issn | 1699-5848 | - |
dc.identifier.uri | http://hdl.handle.net/10201/134425 | - |
dc.description.abstract | Objective. To investigate the effects of Hyperin (Hyp) on osteogenic differentiation of MC3T3E1 cells. Methods. Differentially expressed miRNA was screened by miRNA Microarray. miR-7031-5P overexpression and knockdown MC3T3-E1 cell models were constructed by transfecting miR-7031-5P mimics and inhibitor. Alizarin red staining (ARS) assay was used to observe the formation of mineralized nodules in MC3T3-E1 cells. ALP activity was detected by using ALP detection kit. Western blot assay was used to examine the changes in osteogenic differentiation-related proteins. The relationship between miR-7031-5P and Wnt7a was revealed by dual luciferase report experiments. Results. We found that miR-7031-5P was upregulated in MC3T3-E1 cells after Hyp treatment. The results indicated that compared with the untreated group, Hyp promoted the formation of mineralized nodules and the alkaline phosphatase (ALP) activity of MC3T3-E1 cells via overexpressing miR-7031-5P. Besides, elevated miR-7031-5P increased OPN, COL1A1, and Runx2 mRNA expression. More importantly, Wnt7a was identified as the downstream target gene of miR-70315P promoting osteogenic differentiation of MC3T3-E1 cells. Conclusions. Hyp up-regulated miR-7031-5P to promote osteogenic differentiation of MC3T3-E1 cells by targeting Wnt7a | es |
dc.format | application/pdf | es |
dc.format.extent | 11 | es |
dc.language | eng | es |
dc.publisher | Universidad de Murcia, Departamento de Biologia Celular e Histiologia | es |
dc.relation | Sin financiación externa a la Universidad | es |
dc.rights | info:eu-repo/semantics/openAccess | es |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Hyp | es |
dc.subject | MC3T3-E1 | es |
dc.subject | Wnt7a | es |
dc.subject | Osteogenic differentiation | es |
dc.subject | miR-7031-5P | es |
dc.subject.other | CDU::6 - Ciencias aplicadas::61 - Medicina::616 - Patología. Medicina clínica. Oncología | es |
dc.title | Hyperin up-regulates miR-7031-5P to promote osteogenic differentiation of MC3T3-E1 cells | es |
dc.type | info:eu-repo/semantics/article | es |
dc.identifier.doi | https://doi.org/10.14670/HH-18-579 | - |
Aparece en las colecciones: | Vol.38,nº10 (2023) |
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