Por favor, use este identificador para citar o enlazar este ítem:
https://doi.org/10.14670/HH-18-538
Twittear
Registro completo de metadatos
Campo DC | Valor | Lengua/Idioma |
---|---|---|
dc.contributor.author | Zhang, Na | - |
dc.contributor.author | Li, Jing | - |
dc.contributor.author | Sun, Huapeng | - |
dc.contributor.author | Tian, Aixia | - |
dc.contributor.author | Chen, Yuhua | - |
dc.date.accessioned | 2023-09-07T10:51:08Z | - |
dc.date.available | 2023-09-07T10:51:08Z | - |
dc.date.issued | 2023 | - |
dc.identifier.citation | Histology and Histopathology Vol. 38, nº8 (2023) | es |
dc.identifier.issn | 0213-3911 | - |
dc.identifier.issn | 1699-5848 | - |
dc.identifier.uri | http://hdl.handle.net/10201/133712 | - |
dc.description.abstract | Background. Gallbladder carcinoma (GBC) is a common cancer disease with high mortality. Circular RNA_0008234 (circ_0008234) has been shown to play a key role in many tumors, including GBC. However, the function between circ_0008234 and microRNA-204-5p (miR-204-5p) in the progression of GBC has not been clarified. Methods. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to detect the expressions of circ_0008234, miR-204-5p and fibroblast growth factor receptor-2 (FGFR2) in GBC cells and tissues. Western blot was used to detect the expression of relative proteins. Cell proliferation, apoptosis, invasion and migration were detected by 3-(4, 5-dimethylthiazol2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay, thymidine analog 5-ethynyl-2’-deoxyuridine (EdU) assay, flow cytometry, transwell assay and wound healing assay. Mechanically, the interaction of miR-2045p with circ_0008234/FGFR2 was notarized by dualluciferase reporter assay. A xenotransplantation model was established to study the role of circ_0008234 in vivo. Results. Circ_0008234 and FGFR2 were highly expressed in GBC tissues and cells. Silencing circ_0008234 down-regulated cell proliferation, migration and invasion of NOZ and SGC-996 cells, while miR-204-5p inhibitors reversed these effects. In addition, overexpression of FGFR2 restored the cell malignant behavior of GBC cells inhibited by miR-2045p mimic. Animal experiments confirmed the anti-tumor effect of silenced circ_0008234 in vivo. Conclusion: Circ_0008234 mediated GBC via the miR-204-5p/FGFR2 axis, providing a novel targeted therapy for gallbladder carcinoma | es |
dc.format | application/pdf | es |
dc.format.extent | 13 | es |
dc.language | eng | es |
dc.publisher | Universidad de Murcia, Departamento de Biologia Celular e Histiologia | es |
dc.relation | Sin financiación externa a la Universidad | es |
dc.rights | info:eu-repo/semantics/openAccess | es |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Circ_0008234 | es |
dc.subject | miR-204-5p | es |
dc.subject | FGFR2 | es |
dc.subject | Gallbladder carcinoma | es |
dc.subject.other | CDU::6 - Ciencias aplicadas::61 - Medicina::616 - Patología. Medicina clínica. Oncología | es |
dc.title | Circ_0008234 regulates the biological process of gallbladder carcinoma by targeting the miR-204-5p/FGFR2 axis | es |
dc.type | info:eu-repo/semantics/article | es |
dc.identifier.doi | https://doi.org/10.14670/HH-18-538 | - |
Aparece en las colecciones: | Vol.38, nº8 (2023) |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
35632a5a-2e5c-429c-9381-55757b97c843.pdf | 8,45 MB | Adobe PDF | Visualizar/Abrir |
Este ítem está sujeto a una licencia Creative Commons Licencia Creative Commons