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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Hu, Sizhe | - |
dc.contributor.author | Zhou, Huanting | - |
dc.contributor.author | Zhao, Xiaokang | - |
dc.contributor.author | Qian, Feng | - |
dc.contributor.author | Jin, Cancan | - |
dc.date.accessioned | 2023-05-24T15:55:58Z | - |
dc.date.available | 2023-05-24T15:55:58Z | - |
dc.date.issued | 2023 | - |
dc.identifier.citation | Histology and Histopathology Vol. 38, nº6 (2023) | es |
dc.identifier.issn | 0213-3911 | - |
dc.identifier.issn | 1699-5848 | - |
dc.identifier.uri | http://hdl.handle.net/10201/131392 | - |
dc.description.abstract | Gastric adenocarcinoma (GAC) caused by malignant transformation of gastric adenocytes is a malignancy with high incidence. MiR-195-5p modulates a variety of cancers. One of its target genes, orthodenticle homeobox 1 (OTX1), is believed to be a key modulator of tumor progression. We aim to analyze the mechanism of miR-195-5p and OTX1 in GAC. MiR195-5p and OTX1 mRNA levels in GAC cells were tested via qRT-PCR. OTX1 protein and EMT-related protein levels were examined through western blot. Several cell functional assays were designed to measure changes in cell malignant behaviors. Dual luciferase assay verified the targeting relation of miR-195-5p and OTX1. These experimental results showed significantly low miR-195-5p expression and significantly high OTX1 expression in GAC cells. Enforced miR-195-5p level repressed cell malignant progression and accelerated cell apoptosis in GAC. Increased OTX1 weakened the above-mentioned effect caused by overexpressing miR-195-5p. Thus, miR-195-5p restrained migration, proliferation, invasion and epithelial-mesenchymal transition process of GAC cells, and promoted cell apoptosis through regulating OTX1. A new insight is provided for searching for biomarkers or therapeutic targets of GAC. | es |
dc.format | application/pdf | es |
dc.format.extent | 10 | es |
dc.language | eng | es |
dc.publisher | Universidad de Murcia, Departamento de Biologia Celular e Histiologia | es |
dc.relation | Sin financiación externa a la Universidad | es |
dc.rights | info:eu-repo/semantics/openAccess | es |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | miR-195-5p | es |
dc.subject | Gastric adenocarcinoma | es |
dc.subject | OTX1 | es |
dc.subject | Invasion | es |
dc.subject | Migration | es |
dc.subject | Proliferation | es |
dc.subject | Epithelialmesenchymal transition | es |
dc.subject.other | CDU::6 - Ciencias aplicadas::61 - Medicina::616 - Patología. Medicina clínica. Oncología | es |
dc.title | MiR-195-5p suppresses gastric adenocarcinoma cell progression via targeting OTX1 | es |
dc.type | info:eu-repo/semantics/article | es |
dc.identifier.doi | https://doi.org/10.14670/HH-18-512 | - |
Aparece en las colecciones: | Vol.38, nº6 (2023) |
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Fichero | Descripción | Tamaño | Formato | |
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Hu-38-659-668-2023.pdf | 7 MB | Adobe PDF | Visualizar/Abrir |
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