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https://doi.org/10.14670/HH-18-516
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Título: | Circular RNA circ-CD44 regulates chemotherapy resistance by targeting the miR-330-5p/ABCC1 axis in colorectal cancer cells |
Fecha de publicación: | 2023 |
Editorial: | Universidad de Murcia, Departamento de Biologia Celular e Histiologia |
Cita bibliográfica: | Histology and Histopathology Vol. 38, nº2 (2023) |
ISSN: | 0213-3911 1699-5848 |
Materias relacionadas: | CDU::6 - Ciencias aplicadas::61 - Medicina::616 - Patología. Medicina clínica. Oncología |
Palabras clave: | circ-CD44 miR-330-5p ABCC1 CRC OXA |
Resumen: | . Background. Colorectal cancer (CRC) is a common malignant tumor worldwide, ranking fourth for incidence. Recently, circular RNAs (circRNAs) have been demonstrated to play a key role in chemotherapy resistance to CRC treatment. Therefore, the role of circCD44 is investigated in CRC. Methods. The expression levels of circ-CD44, miR330-5p, and ATP binding cassette subfamily C member 1 (ABCC1) were quantified by real-time quantitative polymerase chain reaction (RT-qPCR) assay. The sensitivity of CRC cells to oxaliplatin (OXA) was assessed by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl2H-tetrazol-3-ium bromide (MTT) assay. Colonyforming experiment was performed to measure the colony-forming ability of CRC cells. The apoptosis, migration, and invasion of CRC cells were determined by flow cytometry and transwell assays. A xenograft experiment was established to clarify the functional role of circ-CD44 silencing in vivo. The interactional relationship among circ-CD44, miR-330-5p, and ABCC1 was confirmed by dual-luciferase reporter and RNA immunoprecipitation assays. The protein expression of ABCC1 was quantified by western blot assay. Results. Circ-CD44 was obviously upregulated in OXA-resistant colorectal cancer tissues and cells. Lossof-function experiments revealed that inhibition of circCD44 suppressed proliferation, migration, and invasion while it increased OXA sensitivity and apoptosis in OXA-resistant colorectal cancer cells, which was overturned by suppression of miR-330-5p; besides, silencing of circ-CD44 also slowed the tumor growth in vivo. Additionally, overexpression of miR-330-5p inhibited chemotherapy resistance, proliferation, migration, and invasion while it induced apoptosis by targeting ABCC1. Conclusion. Mechanistically, circ-CD44 functioned as a miRNA sponge for miR-330-5p to upregulate the expression of ABCC1 and regulate chemotherapy resistance in CRC cells |
Autor/es principal/es: | Zhao, Shuai Xu, Fei Ji, Yiding Wang, Yuanyuan Wei, Ming Zhang, Like |
URI: | http://hdl.handle.net/10201/129328 |
DOI: | https://doi.org/10.14670/HH-18-516 |
Tipo de documento: | info:eu-repo/semantics/article |
Número páginas / Extensión: | 13 |
Derechos: | info:eu-repo/semantics/openAccess Attribution-NonCommercial-NoDerivatives 4.0 Internacional |
Aparece en las colecciones: | Vol.38, nº2 (2023) |
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Fichero | Descripción | Tamaño | Formato | |
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Zhao-38-209-221-2023.pdf | 7,7 MB | Adobe PDF | ![]() Visualizar/Abrir |
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