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https://doi.org/ 10.14670/HH-18-508
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Campo DC | Valor | Lengua/Idioma |
---|---|---|
dc.contributor.author | Tan, Shengquan | - |
dc.contributor.author | Hu, Lingbo | - |
dc.contributor.author | Lei, Rui | - |
dc.contributor.author | Wang, Ruo | - |
dc.contributor.author | Chen, Jiaquan | - |
dc.date.accessioned | 2023-03-13T09:27:07Z | - |
dc.date.available | 2023-03-13T09:27:07Z | - |
dc.date.issued | 2023 | - |
dc.identifier.citation | Histology and Histopathology Vol. 38, nº2 (2023) | es |
dc.identifier.issn | 0213-3911 | - |
dc.identifier.issn | 1699-5848 | - |
dc.identifier.uri | http://hdl.handle.net/10201/129325 | - |
dc.description.abstract | Background. Gastric cancer (GC) ranks fourth as a cause of cancer-induced mortality worldwide. Recently, some studies have demonstrated that circular RNAs (circRNAs) play vital roles in human cancers, including GC. Methods. The expression levels of circ_0000467, microRNA-622 (miR-622), and Rho-associated coiledcoil-containing protein kinase2 (ROCK2) were determined by RT-qPCR assay. The protein expression was quantified by western blot assay. The interaction relationship between miR-622 and circ_0000467 or ROCK2 was confirmed by dual-luciferase reporter assay and RIP assay. The biological behaviors of GC cells including proliferation, apoptosis, migration, and invasion were determined by EdU assay, colony-forming assay, flow cytometry, and transwell assay. The effects of circ_0000467 silencing in vivo were assessed by a xenograft experiment in nude mice. Results. MiR-622 was downregulated and ROCK2 was upregulated in GC tissues and cells. Loss-offunction experiment revealed that overexpression of miR-622 decreased proliferation, migration, and invasion while it increased apoptosis in GC cells. Furthermore, ROCK2 was a functional target of miR622, and upregulation of ROCK2 abolished miR-622- induced effects on GC cells. What’s more, circ_0000467 was upregulated in GC, and inhibition of miR-622 reversed silencing of circ_0000467-caused effects on GC cells, suggesting that miR-622 was a target of circ_0000467. The suppression of circ_0000467 was able to slow the tumor growth in vivo. Conclusion. Mechanistically, circ_0000467 functioned as an oncogenic regulator in GC by specifically binding to miR-622 to upregulate ROCK2, which might be novel diagnostic markers for GC. | es |
dc.format | application/pdf | es |
dc.format.extent | 13 | es |
dc.language | eng | es |
dc.publisher | Universidad de Murcia, Departamento de Biologia Celular e Histiologia | es |
dc.relation | Sin financiación externa a la Universidad | es |
dc.rights | info:eu-repo/semantics/openAccess | es |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Gastric cancer | es |
dc.subject | circ_0000467 | es |
dc.subject | miR-622 | es |
dc.subject | ROCK2 | es |
dc.subject.other | CDU::6 - Ciencias aplicadas::61 - Medicina::616 - Patología. Medicina clínica. Oncología | es |
dc.title | Circ_0000467 regulates proliferation, migration, invasion, and apoptosis in gastric cancer by targeting the miR-622/ROCK2 axis | es |
dc.type | info:eu-repo/semantics/article | es |
dc.identifier.doi | https://doi.org/ 10.14670/HH-18-508 | - |
Aparece en las colecciones: | Vol.38, nº2 (2023) |
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Tan-38-185-197-2023.pdf | 14,16 MB | Adobe PDF | ![]() Visualizar/Abrir |
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