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https://doi.org/10.14670/HH-18-507
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Campo DC | Valor | Lengua/Idioma |
---|---|---|
dc.contributor.author | Dong, Shifang | - |
dc.contributor.author | Zhong, Haiying | - |
dc.contributor.author | Li, Lin | - |
dc.date.accessioned | 2023-03-13T09:19:28Z | - |
dc.date.available | 2023-03-13T09:19:28Z | - |
dc.date.issued | 2023 | - |
dc.identifier.citation | Histology and Histopathology Vol. 38, nº2 (2023) | es |
dc.identifier.issn | 0213-3911 | - |
dc.identifier.issn | 1699-5848 | - |
dc.identifier.uri | http://hdl.handle.net/10201/129306 | - |
dc.description.abstract | Background. Acute myeloid leukemia (AML) is a malignant hematological neoplasm in adults. Researche indicates that circular RNAs (circRNAs) play paramount roles in the pathological process of AML. In this study, the role of circ_DLEU2 (circ_0000488) in AML is revealed. Methods. The expression of circ_DLEU2, microRNA-582-5p (miR-582-5p) and cyclooxygenase 2 (COX2) was determined by quantitative real-time PCR. Protein expression was detected by western blot. Cell proliferation was investigated by cell cycle, 5-Ethynyl29-deoxyuridine and 3-(4,5)-dimethylthiahiazo (-z-y1)- 3,5-di-phenytetrazoliumromide (MTT) assays. Cell apoptosis was elucidated by apoptosis analysis assay. The targeting relationship between miR-582-5p and circ_DLEU2 or COX2 was predicted by the starbase online database, and identified by a dual-luciferase reporter assay. Results. Circ_DLEU2 and COX2 expression were substantially up-regulated, while miR-582-5p was downregulated in AML marrow samples and cells compared with control groups. Circ_DLEU2 knockdown suppressed cell proliferation, whereas it induced cell arrest at G0/G1 phase and cell apoptosis in AML; however, these effects were attenuated by miR-582-5p inhibitor. Additionally, circ_DLEU2 was associated with miR-582-5p, and miR-582-5p bound to COX2 in AML cells. Also, we found that circ_DLEU2 regulated COX2 expression by interacting with miR-582-5p. Conclusion. Circ_DLEU2 silencing hindered AML malignant progression via downregulating COX2 through sponging miR-582-5p. Our finding provides a theoretical basis for studying circRNA-directed therapy of AML | es |
dc.format | application/pdf | es |
dc.format.extent | 13 | es |
dc.language | eng | es |
dc.publisher | Universidad de Murcia, Departamento de Biologia Celular e Histiologia | es |
dc.relation | Sin financiación externa a la Universidad | es |
dc.rights | info:eu-repo/semantics/openAccess | es |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | AML | es |
dc.subject | circ_DLEU2 | es |
dc.subject | miR-582-5p | es |
dc.subject | COX2 | es |
dc.subject.other | CDU::6 - Ciencias aplicadas::61 - Medicina::616 - Patología. Medicina clínica. Oncología | es |
dc.title | Circ_DLEU2 knockdown represses cell proliferation, migration and invasion, and induces cell apoptosis through the miR-582-5p/COX2 pathway in acute myeloid leukemia | es |
dc.type | info:eu-repo/semantics/article | es |
dc.identifier.doi | https://doi.org/10.14670/HH-18-507 | - |
Aparece en las colecciones: | Vol.38, nº2 (2023) |
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Dong-38-171-183-2023.pdf | 9,56 MB | Adobe PDF | ![]() Visualizar/Abrir |
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