MiR-194-3p modulates the progression of colorectal cancer by targeting KLK10

Abstract

Background. A rich history of studies have manifested the importance of miRNAs to cancer progression, while miR-194-3p has been seldom explored. Objective. The purpose of this study is to unearth the way the miR-194-3p/KLK10 axis modulates colorectal cancer (CRC). Methods. Differentially expressed genes of CRC in TCGA database were analyzed. Western blot and qRTPCR were employed to test protein and mRNA expressions of two researched genes. Their targeting was confirmed using dual-luciferase. Biological behaviors of cells were tested by a series of cellular functional assays. Result. Remarkably low miR-194-3p expression and high KLK10 expression were observed in cancer cells. Overexpressing miR-194-3p hindered the progression of CRC cells. Overexpression of miR-194-3p significantly weakened the promoting effect of upregulated KLK10 on cell migration, invasion and proliferation. Their targeting was verified by dual -luciferase assay. Therefore, miR-194-3p hindered cell behaviors of CRC through KLK10. Conclusion. This investigation casts new light on the treatment of CRC through the miR-194-3p/KLK10 axis.